Preparation Of Nano Surface Coating Of Titanium And Its Biological Evaluation

Titanium is currently the most widely used in clinical human implant material, but pure titanium material is a biologically inert material, after the initial difficult to form implanted early osseointegration, low initial bond strength with the surrounding bone tissue. Therefore, the most talked about current research is to improve the early post-implant osseointegration. rhBMP-2(human bone morphogenetic protein-2) has been shown to increase cell osteogenic differentiation. TiO2surface nanostructures have been shown to improve the performance of osteoblast cells, and can promote osteoblast differentiation and proliferation. But its surface features make it not possible for the protein molecules carrying and sustained release.In this study, MBG (mesoporous bioactive glass) has a strong bearing capacity and slow-release, which combines with TiO2nanorods build a new type of morphology, namely:height adjustable nanorods were exposed, and has strong bearing capacity and sustained release of macromolecular substances. Which nanorods highly exposed to varying degrees by embedding MBG range can be controlled within the range of lOnm-300nm.After this new structure for the evaluation and analysis of biology, the results of the experiment show that the structure of TiO2nanorod structure than a single MBG and TiO2nanorods have a stronger combination of biocompatibility, a former front proliferation of bone cells can reach1.5times the latter. While carrying MBG rhBMP-2in/TiO2film structure compared to the structure of rhBMP-2is not significantly improved bearing BMSCs (bone marrow mesenchymal stem cells) osteogenic differentiation and mineralization can be achieved by about2-fold and1.3respectively times.This study showed that MBG/TiO2film structure surface and TiO2nanorods MBG constructed combining to increase its biological activity. Similar to the natural extracellular matrix biomimetic three-dimensional living space, can induce cell filopodia have better adhesion. Hosted by rhBMP-2can accelerate osteogenic differentiation of cells. This can provide an effective reference for improving the early post-implant osseointegration.