Expression And Function Study Of GOLPH3in Non-small-cell Lung Cancer

Objective:To investigate the relevance of Golgi phosphoprotein3(GOLPH3) to Non-small-cell lung cancer(NSCLC).Methods:The expression of GOLPH3in NSCLC and matched adjacent tissues was determined by immunohistochemistry (IHC).The association of GOLPH3expression with clinicopathological features and overall suvival was analyzed. The biological relevance of GOLPH3was explored by MTS assay and other approaches such as flow cytometry analysis.Results:GOLPH3expression was significantly elevated in NSCLC tissues compared to adjacent non-tumor tissues (χ2=15.172, P<0.01). GOLPH3expression was significantly different in various groups of histological types (χ2=12.98,P<0.01), differentiation (χ2=36.14,P<0.01), and lymph node metastasis (χ2=4.71,P<0.05). Kaplan-Meier Survival analysis showed that the overall survival of patients with high GOLPH3expression were significantly shorter than those with low GOLPH3expression. Western blotting and RT-PCR Showed GOLPH3was highly expressed in NSCLC cell lines. After knocking down GOLPH3using SiRNA, cell proliferation were decreased accompanied, increased apoptosis and cell cycle arrest in G2-M-phase. Conclusions:By affecting cell proliferation, GOLPH3is closely related to the progression of NSCLC and could be served as a novel prognostic biomarker for NSCLC patients.