Mimic Epitope Of Ochratoxin A And Its Application In Phage ELISA

The donkey anti-mouse IgG was firstly coated onto the well of plates, and then anti-ochratoxin A monoclonal antibodies(anti-OTA mAbs) added to bind with the donkey anti-mouse IgG were used to select the mimic epitope of OTA antigen. The enzyme-linked immunosorbent assay(ELISA) for OTA was developed based on the obtained filamentous phage. The results showed that the amino acid sequence that can mimic OTA was GMSWMMA. The proposed phage ELISA exhibited a dynamic linear detection of OTA from 0.03 ng/mL to 0.50 ng/mL with a half inhibition concentration(IC50) of 0.145 ± 0.018 ng/mL. The recoveries with OTA spiked rice samples for intra-assay ranged from 96.95%~115.20% whereas that for inter-assay were 107.23%~123.13%. Meanwhile, the results of OTA spiked real rice samples obtained by phage ELISA and commercial ELISA exhibited no significant difference. All in all, the proposed phage ELISA offers great potential for rapid and sensitive detection of OTA in food safety.