| Objective: Detection of CD133+ in gastric cancer stem cells express further explore CD133+cells were distributed in gastric cancer, morphological characteristics, the number of its significance; detect cancer stem cell marker CD133+ Gastric understand cancer stem cell marker CD133+ development of gastric cancer relationship with CD133+cancer stem cells as a marker of gastric value.Methods:Mechanically isolated primary cultured gastric cancer stem cells observed morphology; using MACS (magnetic activated cell sorting) isolated and cultured gastric CD133+ cancer stem cells were gastric CD133+ cancer stem cell spheres form; CD 133+expression by flow cytometry gastric cancer stem cells rate.Results:1. Mechanical Separation primary cultured gastric cancer stem cells found in cell morphology by small and round.2. CD133+cells in MACS method can be isolated and cultured in suspension growth, proliferation, and with increasing incubation time, the cells gradually gathered, while the formation of tumor cell suspension ball.3. CD 133+positive cells was screened prior to 5.05 percent, after the screening of cells positive rate was 88.07%, the positive rate was significantly higher than that of cells screened before screening.4.The article points to a fresh 5×104 selected CD133+ and CD 133- were injected subcutaneously into nude armpit, divided into positive group (CD133+) and negative group (CD 133-), n=6.82.5% positive tumor group (5/6), negative group 16.67%(1/6). Table 1 shows the results, CD 133+tumor rate and tumor size were higher than CD 133".5.After the tumor tissue by HE staining can be observed scattered on CD133+ expression and cell membrane.Conclusion:CD133+ is a surface marker of gastric cancer stem cells for expression of CD133+ in gastric cancer detection, help assess the biological behavior of the tumor; CD133+ as a valid marker of gastric cancer stem cells. |
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