The Study Of The Expression Of MiR-125a-3p、miR-193b-3p In Peripheral Blood Mononuclear Cells Of Coronary Heart Disease Patients

Objective:To detect the expression level of miR-125a-3p, miR-193b-3p in peripheral blood mononuclear cells of Coronary atherosclerotic heart disease(CHD) patients, and investigated the correlation of those with Coronary heart disease for the further study of diagnosis and treatment of CHD.Methods:63 patients with Coronary heart disease and 20 normal control patients were collected in the Kunming Yan’an hospital from August 1,2014 to October 30,2014. The patients with coronary heart disease were divided into 23 cases of stable angina pectoris group, and 40 cases of acute coronary syndrome group. All people were confirmed by coronary angiography. Extracting object venous blood and separating the mononuclear cells, total RNA was extracted from the resulting mononuclear cells, and then the total RNA was reverse transcribed according to the kit instructions using the reverse transcription object microRNA reverse primer synthesis, the level of miRNAs in blood was detected by using real-time fluoresecnt quantitative PCR. To detect the expression level of peripheral blood mononuclear cells differentiation miR-125a-3p 、miR-193b-3p in patients of Coronary atherosclerotic heart disease(CHD). Statistical analysis was performed by using SPSS 17.0 software.Results:1. The expression level of miR-125a-3p and miR-193b-3p in blood were different in the group A (Normal control group), group B (Stable angina pectoris group), group C (Acute coronary syndrome group) (P<0.01).2. Compared with group A, the levels of miR-125a-3p in group B、group C were significant high expression (P<0.01).Compared with group B, the levels of miR-125a-3p in group C was significant high expression (P<0.01).3. Compared with group A, the levels of miR-193b-3p in group B、group C were significant high expression (P<0.01).Compared with group B, the levels of miR-193b-3p in group Cwas significant high expression (P<0.01).Conclusions:1.The expression level of mononuclear cells differentiaion miR-125a-3p in the patients with coronary atherosclerotic heart disease have significantly increased,and Acute coronary syndrome group> Stable angina pectoris group> Normal control group.2.The expression level of mononuclear cells differentiaion miR-193b-3p in the patients with coronary atherosclerotic heart disease have significantly increased,and Acute coronary syndrome group> Stable angina pectoris group> Normal control group.