Effect Of Static Magnetic Fields On The Biological Charasteristics Of Mandibular Condylar Chondrocyte And Nasal Septum Chondrocyte

[Objective] Set up SD rat MCC (mandibular condylar chondrocytes, MCC) and NSC (nasal septal chondrocyte, NSC) in vitro models through the static magnetic fields,observes the difference of biological characteristics of MCC and NSC morphological by the microscope after the static magnetic fields expose.For more in-depth study of theoretical basis in maxillofacial growth mechanism in the development proceess differences in biological characteristics of MCC and NSC in the static magnetic fields,the following provides guidance.[Methods] The condylar cartilage and nasal septal chondrocyte cells of new born SD rats which were born in one to three days,were taken in vitro after mechanicalseparation combined with enzymatic digestion.They were cultured in the same culture conditions.Observes the MCC and NSC cells morphology and growth conditions by the microscope after culturing under 160mT,280mT and 360mT static magnetic fields.Compare with each group and select the most efficient field strengh by MTT.Chondrocytes phenotype identification were examined by Type Ⅱ collagen immunohistochemical staining and toluidine blue staining to observe the difference of the two cartilage extracellular matrix synthetsis.We detect the expression differences of type Ⅱ collagen (type Ⅱ collagn) and AGG mRNA with RT-PCR (real time quantitative polymerase chain reaction, RT-PCR) after the static magnetic fields expose.[Results] Use the same digesting method and culture conditions to acquire MCC and NSC.They underwent 160mT,280mT and 360 mT static magnetic fields after most cells attachmet.Choose 280mT static magnetic fields which encourage cell proliferation significantly as the best condition by MTT assay,set up the control group at the same time.We found the positive expression on day 3,5,8 by the Type Ⅱ collagen immunohistochemical staining and toluidine blue staining.We also test the expression of collagen Ⅱ and AGG mRNA on day 3,5,8 by RT-PCR.The result is that two group with static magnetic fields were stronger than control group in all assay.At the same time points,NSC were stronger than MCC.[Conclusions] 1.In this study,Use the same digesting method and culture conditions to primary the model of MCC and NSC cultured under static magnetic fields successfully.2.In the 160,280,360mT of static magnetic fields,280mT is the best to promot cell proliferation.it shows that appropriate static magnetic field strength can promote the proliferation of chondrocytes.3.The expression of collagen Ⅱ and AGG mRNA,the synthesis of GAG that MCC and NSC cultured after the static magnetic field,all those changes are similar with the expression of embryonic cartilage formation process.the static magnetic field can promote the expression of collagen II, AGG mRNA and GAG.At the same time,NSC were stonger than MCC.It can affect both cartilage extracellular matrix synthesis by loading a static magnetic field,thus affecting the biological characteristics of both.MCC and NSC differ on the reaction of the static magnetic field,which may be closely related to the mechanism and biological characteristics of the formation of them.