Study On The Correlation Of Simulated Body Fluid Mineralization Products And Osteoblast Behavior

Bone defects brought great inconvenience to patients. Aautologous bone graft is easy to cause secondary damage, immune rejection. With the development of tissue engineering, bone tissue engineering makes the construction of artificial bone the solution to above problems. Based on the materia with high biocompatibility, by electrospinning the scaffold with structure similar to extracellular matrix can give higher biocompatibility. In order to improve the performance of artificial bone, the researchers put hydroxyapatite (HA), three of calcium phosphate (P-TCP) particles of inorganic ceramic particles into the scaffold, and achieved the desired results. Nano ceramic particles is difficult to disperse uniformly in polymer solution for electrospinning due to the dry powder form, and after ultrasonic dispersion and surface modification of nanoparticles, the reunion phenomenon still exists. Biomineralization is initially as a means of performance evaluation of bone repair material. Bone repair material with good properties can form a uniform layer of calcium and phosphorus in simulated body tluid, the dispersion effect is better than that of the inorganic ceramic particles directly dispersed into the spinning solution.Simulated body fluid is used as different types for researchers, with the different surface of polymer materials, and ultimately may have been promoting bone cell differentiation. However, there are differences caused by the different mineralization time and other factors in mineralization. There are differences in the microstructure composition, structure, and growth process in cell culture medium for the apatite above, and these differences will effect the osteogenic differentiation of cells. When researchers focus on mineralization products, they pay little attention to product characteristics of each stage in mineralization.We choose poly-L-lactic acid/gelatin as polymer matrix prepared by electrospinning. We choose modified i-5SBF as mineralization system because of the short mineralization time and mineralization products changed significantly. With the mineralization process, we pick mineralized matrix off the mineralization system every 6 hours. In order to study the influence of mineralization in the process of dissolution in cell culture, excluding the effects of proteins and other organic matters, we choose HANKS buffer solution as the soaking system, compared with deionized water as control soaking system. Similar to cell culture, the soaking solution was refreshed at day 1,3,6,9,12,15,18, and the material was removed from the dissolution system characterized by SEM, XRD, FT-IR, weighing,ICP.Osteoblasts were cocultured with mineralized product with different stages to osteoblast proliferation, ALP activity, effect of type I collagen synthesis. Osteoblasts were cocultured with mineralized product in Transwell devices to test the proliferation and activity of ALP. From in vitro dissolution experiments and cell culture experiments, we can get not only the products at different mineralization stages effecting on the cells, but also can provide a evaluation model for other mineralization system.