| Objective: The author proposes an approach to improve the treatment of common acne by baicalin associated with acne clearance technique.Methods:(1) To establish the main components of the formulation baicalin, menthol methodology. Skullcap baicalin content was determined by HPLC, Mint oil menthol content was determined by GC.(2) Examined the impact of different penetration enhancers on percutaneous penetration of baicalin effect. Comparison of different concentrations of Azone,peppermint volatile oil of baicalin transdermal drug amount, and then investigate the best dosage of the penetration enhancers in determining Transdermal dosage, the study dosage baicalin percutaneous penetration effect impact.(3) Study the best prescription baicalin gel. By gel quality control methods, use the indicators of the viscosity and spreadability,by the L9(34) orthogonal test gel formulation were optimized to determine the best recipe of the baicalin gel; after using in vitro, to test the effects of transdermal method mint volatile oil baicalin percutaneous penetration of drug effects.(4) The use of an optical microscope affect penetration enhancers peppermint volatile oil and Drug Baicalin on the skin of rats under the cuticle microstructure.(5) According to MIC susceptibility test regulations from the US Committee forClinical Laboratory Standards pot(NCCLS) regularly published, examining baicalin gel antibacterial activity of Propionibacterium acnes. Through filter paper method and investigated 96 law baicalin gel Propionibacterium acnes inhibition.(6) The use of rabbit ear model baicalin acne gel a preliminary evaluation of efficacy.By smearing black tar rabbit ear ear canal opening 2cm*2cm at the first seven days in modeling correction using sterile saline to 1*108CFU/ml solution of Propionibacterium acnes subcutaneously in the rabbit ear lesions at each side rabbit ears were about 50 ul modeling, in modeling positive drug investigation 28 days fusidic acid cream and gel baicalin rabbit ears acne treatment.Results:(1) Measured by HPLC method Baicalin precision RSD was: 0.28% RSD for reproducibility: 1.94%, and the recovery is: 100.58%, RSD of the measured gas chromatographic method for menthol: 1.86 % RSD for reproducibility: 1.38%, and the recovery is: 99.29%, indicating that the results of the two methods of measuring a good, stable and feasible.(2) 3% mint oil as the penetration enhancers Baicalin permeation rate(Js) is 0.202μg.(cm-2.h-1), slightly higher than 0.198μg 3% Azone’s.(cm-2.h-1), 1.5% + 1.5% Azone mint volatile oil associated with 0.111μg.(cm-2.h-1), so the choice of 3% mint volatile oil as penetration enhancers. Separate study mint volatile oil concentration, 3%concentration of Baicalin permeation rate(Js) is 0.0903μg.(cm-2.h-1) is higher than the concentration of 0.04μg 2%.(cm-2.h-1), 0.0393μg 1% concentration in.(cm-2.h-1).Under the same circumstances mint oil, 1% concentration of Baicalin 0.1996μg.(cm-2.h-1) was significantly higher than 0.5%, 0.75%, 1.5%, 2% penetration rate of Baicalin concentration, so the final selection criteria of 3% mint volatile oil as penetration enhancers, Baicalin amount of 1%.(3) The use of L9(34) orthogonal test recipe gel formulations were optimized, the best preparation Baicalin gel is: take the right amount of Carbopol-940 sprinkle the right amount of distilled water, standing, overnight to fully swell. Glycerol,1,3-propanediol dissolved right amount of distilled water, then add the mint volatile oil stir, pour the swelling has fully carbomer-940, and set aside. Another prescription Baicalin powder, stir to dissolve. To join a matrix with a good, stir, add 10% sodiumhydroxide solution to adjust the pH value within the specified range, add distilled water quantitatively to 100 g. Baicalin gel Js for 0.013μg.(cm-2.h-1), is 2.40 times the penetration without promoting agent, indicating gels Baicalin percutaneous penetration rate is good.(4) In the structure of the skin of rats under the peppermint essential oil, change the order of the compact structure of the stratum corneum, epidermis structure becomes loose, reducing the barrier function of the skin.(5) Filter paper method in experiments Baicalin gels inhibition zone level within a range of 3 blank was no inhibition zone, metronidazole group inhibition zone for three,ampicillin group has antibacterial circles, for four. 96 Experimental Law Baicalin gel MIC value 0.03906mg/g, Baicalin crude extracts MIC value 31.25mg/m L, MIC values ??Baicalin gel is much lower than the crude extract group, acne propionate acid bacillus two antibiotics metronidazole and ampicillin are more sensitive, and no resistance;(6) Baicalin gel and the fusidic acid for acne rabbit ear model experiments have anti-inflammatory effects, and have anti-keratosis role, but comparing the two, the former is more significant for the anti-inflammatory effects, two Anti keratosis actors rather, may be due to the gel itself Baicalin with certain pigment, dye smear after a long period of time easily precipitate.Conclusions:(1) Baicalin gel in Propionibacterium acnes inhibition experiments, the minimum inhibitory concentration of Baicalin gel is much lower than the crude extract Baicalin,experiments show that the gel mint volatile oil in addition to good the promoting effect, there are some antibacterial effect, so the choice of mint volatile oil as penetration enhancers are more reasonable.(2) Baicalin gel and the fusidic acid cream gel with anti-keratosis role is considerable,but the anti-inflammatory effects of Baicalin gel is superior to the latter, fusidic acid cream anti-inflammatory effect is not obvious Baicalin good antibacterial gel in addition to the effect of the elimination of acne, but also be able to soften the skin, the stratum corneum, and the traditional skullcap extract added directly comparedcosmetics, this formulation can after use to reduce pigmentation and in mint volatile oil under the effect of the inhibition of acne better. |
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