The Interventions Effects Of Curcumin Solid Dispersion Against Cadmium-induced Reproductive Toxicity In Male Rats

Objectives The study by the curcumin and soluble polymer materials of polyvinylpyrrolidone(PVP) prepared as solid dispersion to increase its solubility, improve its bioavailability. Explore curcumin solid dispersion could interventions the productive toxicity of cadmium-induced, it will help to providing new clues and ideas for the treatment of male reproductive toxicity caused by cadmium.Methods 1 Preparation of curcumin solid dispersion: the water-soluble carrier material PVP k30 as a carrier were prepared curcumin solid dispersion of three ratios( 1:2, 1:4, 1:6). Measured by solubility and dissolution in-vitro select the best ratio. Used differential scanning calorimetry(DSC) for verification. 2 The interventions effects of curcumin solid dispersion against cadmium-induced reproductive toxicity in male rats: Make 40 healthy SPF male SD rat(weight 180-200 g) be experimental animals. These animals were divided into five groups at random after being fed adaptability for a week. These five groups were blank group, cadmium group, curcumin group(60 mg/kg), curcumin solid dispersion of low-dose group(300 mg/kg) and curcumin solid dispersion of high-dose group(450 mg/kg). Addition to the blank group the remaining group were injected 2.0 mg/kg cadmium chloride by intraperitoneal for 6 times, every other day, exposed to six times, the blank group intraperitoneal injection of 0.9% saline. Infected while gavage, the blank group and cadmium group with an equal volume of 0.9% saline. Once a day for 6 weeks. Detected the cadmium content in tissues, the epididymal sperm counts, energy of superoxide dismutase(SOD), caspase-3 and content of malondialdehyde(MDA). Observed pathological section of testis tissue by hematoxylineosin(HE) staining. Data processing: using statistical software SPSS Statistics V17.0, the data results were representation with( sx ±), P<0.05 the difference was statistically significant.Results 1 Compared with curcumin, the solubility and dissolution of curcumin solid dispersion are obvious increasing. When the curcumin and PVP k30 mass ratio was 1:4, the solubilizing best. Through DSC analysis that the obtained curcumin solid dispersion. 2 The results of curcumin solid dispersion of male reproductive organs in rats exposed to cadmium intervention study: Cadmium rats show the sperm count, activity rates and SODactivity was significantly reduced, the sperm deformity rate and levels of MDA, Caspase-3 are significantly increased, compared with the control group there is a significantly difference(P<0.05). The sperm count, activity rates and SOD activity of administered group are increased. The sperm deformity rate and levels of MDA, Caspase-3 of administered group are decreased. Pathological section results show that the testis seminiferous tubule is damaged and reducing the number of spermatogenic cells in cadmium group, compared to cadmium group the structure is relatively complete set of administration group, cell status of curcumin solid dispersion high-dose group is near the control group.Conclusions Curcumin has the ability to scavenge free radicals, anti-oxidation, in order to improve its poor solubility and low bioavailability shortcomings, which made a solid dispersion. Curcumin solid dispersion of cadmium toxicity in rats of male reproductive organs, such as sperm viability, motility, abnormal rate, testicular oxidative damage and apoptosis, etc. have a good therapeutic effect, and the role of solid dispersion was significantly better than curcumin group. Show that when made of solid dispersion of curcumin, the absorption of curcumin in the body increases, bioavailability was improved,and its pharmacological activity better than before.