Observe The Expression Of Calcium Sensing Receptor( CaSR) And MAPK Pathway Changes In Myocardial Cells Of Epilepsy Rats

Objective To explore the change of the myocardial cells in chronic epileptic rat model and to observe the expression of calcium sensing receptor( CaSR) and mitogen-activated proteinkinase(MAPK) pathway changes in epilepsy rats.Methods To establish a chronic epileptic rat model kindled by pentetrazole( PTZ). Adult male Wistar rats were selected then divided into 2 groups randomly. The 12 rats in PTZ group received intraperitoneal injection of 1% PTZ by 35 mg/kg for consecutive 28 days. The 12 rats in the normal control group(NC) were injected equal volume of saline intraperitoneally to instead of PTZ. The level of plasma creatine kinase(CK), MB isoenzyme of creatine kinase(CK-MB) were measured by ultraviolet spectrophotometry. The myocardial histologic structural changes were observed under the microscope after HE staining.The change of ultrastructure in the myocardium of rats was observed by electron microscopy. The level of CaSR, ERK(extracellular-signal regulatedprotein kinase), p-ERK(protease extracellular-signal regulatedprotein kinase), p-JNK(protease c-Jun amino-terminal kinase)were detected by western blot.Results Compared with normal control group, CK、CK-MB in PTZ group were increased obviously. By echocardiography, rhythm of the heart is increased, the left ventricular wall motion abnormal, E/A<1. HE staining and electron microscopy both showed the cell swell.The change in expression of CaSR, p-JNK increased, ERK decreased and p-ERK had no obviously change.Conclusions The level of CaSR expression increased in chronic epileptic rat model. CaSR activate the expressions of MAPK of the myocardial cells,then influence the cardiac myocyte apoptosis.