Effects Of Lanthanum Oxide Nanoparticles On The Proliferation Of Parimary Osteoblast In Vitro

In this article, the mechanisms of lanthanum oxide nanoparticles on the proliferation of primary osteoblasts were studied. The physical and chemical properties of lanthanum oxide nanoparticles were characterized by SEM, XRD and DLS. The proliferation of osteoblasts was measured by MTT assay. For the purpose to elucidate the relationship between ROS generation and cell damage, cytomembrane integrity, intracellular ROS level, mitochondrial membrane potential, and cell apoptosis rate were then measured. In addition, the influence of the biological effects of nanomaterials was studied after the protein adsorption. The results are showed follows:1. The lanthanum oxide nanoparticles of different morphologies can inhibit the proliferation of osteoblasts follow a dose-dependent and time-dependent manner. Lanthanum oxide nanoparticles with sheet morphology have a higher inhibition effect than that of rod morphology. Lanthanum oxide nanoparticles inhibit the proliferation of osteoblasts through increasing the levels of intracellular reactive oxygen species and reducing the mitochondrial membrane potential.2.After incubated proteins, the influence of lanthanum oxide nanoparticles on osteoblasts is lower. Protein can change the charge of lanthanum oxide nanoparticles, while comparing of the different proteins, the influence of BSA is lower than BFG.3.The fluorescence of protein reduced after reacting with lanthanum oxide nanoparticles. It is obvious that lanthanum oxide nanoparticles can change the fluorescence of protein with a clear dose-dependent and time-dependent manner. The influence of sheet lanthanum oxide nanoparticles is larger than rod lanthanum oxide nanoparticles in the same dose.4.The result of CD show that, lanthanum oxide nanoparticles can change the characteristic peak of protein. The α-helix and β-sheet will reduce with a clear dose-dependent manner. The influence of sheet lanthanum oxide nanoparticles is larger than rod lanthanum oxide nanoparticles in the same dose.