| Under cardiopulmonary bypass during open heart operation, will experience a process of myocardial ischemia reperfusion. Myocardial ischemia reperfusion injury of myocardial ischemia in certain time to restore the blood supply of ischemic myocardium in a series of more severe toxicity, mainly including arrhythmia, myocardial stunning, coronary circulation obstacle and reperfusion myocardial irreversible damage etc.. At present, the mechanism of the injury of the ischemia reperfusion injury has not been fully elucidated, studies show, free radical, calcium overload, myocardial fiber disturbance of energy metabolism, cell apoptosis may participate in the ischemia reperfusion injury. The study found that, the hydrogen can effectively neutralize hydroxyl radicals and peroxynitrite anion. The experiment found that hydrogen can be used as antioxidant for reperfusion injury induced by cerebral ischemia. Research shows, intravenous injection of saturated hydrogen saline on the animal model of heart, intestine and lung injury has obvious protective effect. This study design through the hydrogen injection perfusion liquid, to observe the myocardial tissue malondialdehyde(MDA) content, superoxide dismutase(SOD) activity, left ventricular systolic pressure(LVSP), left ventricular end diastolic pressure(LVEDP) changes, the maximum rate of rise of left ventricular pressure(+dp/dtmax), the internal pressure of left ventricle the maximum rate of decrease(-dp/dtmax) and the changes of myocardial ultrastructure. In order to further myocardial protection under cardiopulmonary bypass and find more effective method, for the saturated hydrogen saline early application to clinical operation to provide adequate experimental material. Objective: To observe the effects of saturated hydrogen saline on isolated rat heart MDA, SOD, to observe the effects of saturated hydrogen saline on isolated rat heart hemodynamics and ultrastructural mechanism. To investigate the protective effect of saturated hydrogen saline on isolated myocardial ischemic reperfusion injury in rat. Methods: 1) 48 rats were randomly divided into experimental group(24 rats) and control group(24 rats).2) the control group was treated with K-R solution, the experimental group adopted K-R solution and saturated hydrogen saline. Removed after processing of rat heart and the establishment of Langendorff model, and the isolated heart perfusion of 10 min at room temperature according to the inverse, exclusion of 20 min, 20 min reperfusion treatment.3) malondialdehyde(malondialdehyde, MDA) and superoxide dismutase(superoxide, dismutase, SOD) content. Detection of left ventricular systolic pressure(LVSP), left ventricular end diastolic pressure(LVEDP), the maximum rate of rise of left ventricular pressure(+dp/dtmax), left ventricular pressure maximum descending rate(-dp/dtmax). After the end of the experiment, take the left ventricular posterior wall tissue under, immediately with 4% glutaraldehyde fixed specimens, observe the ultrastructural organization of myocardial cells changes under electron microscope. Results: MDA increased to control the most significant group and control group during ischemia, ischemia and ischemia reperfusion period and the experimental group had significant difference(P < 0.01, P < 0.05). The control group during ischemia and ischemia, ischemia reperfusion period compared to previous differences are significant(P < 0.05). No significant differences in early period and ischemia reperfusion group, ischemic period. The activity of SOD in experimental group than in the control group increased and has significant difference(P < 0.01). Compared with the control group, the experimental group ischemia and decrease degree perfusion period LVSP reduced significantly, the difference was statistically significant(P <0.05). Control group, ischemia reperfusion period of LVEDP was obviously increased(P < 0.05), the experimental group ischemia reperfusion LVEDP compared with ischemic early without statistical significance, but compared with the control group decreased significantly(P < 0.05). Compared with the control group, the experimental group decreased the degree of ischemia reperfusion period + dp/dt reduced significantly, the difference was statistically significant(P <0.05). Under the electron microscope observation showed the experimental group, the injury of myocardial ultrastructure significantly reduced compared with the control group. Conclusion: Saturated hydrogen saline on myocardial ischemia reperfusion injury has obvious protective effect. Ischemia reperfusion injury, reduce the formation of oxygen free radical, promote the recovery of heart function. A stable hemodynamic, had a significant protective effect on myocardial ultrastructure. |
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