A Preliminary Study Of The Role Of FHL1 In Pulmonary Artery Hypertension Induced By Hypoxia/Inflammation

Part I Expression of FHL1 in the lung of rats with pulmonary arteryhypertension induced by hypoxia/inflammationObjective: To investigate the expression of FHL1 and paxillin and the role in the occurrence of pulmonary vascular remodeling under the condition of hypoxia/inflammation.Method: 40 male Wistar rats were randomly divided into four group, ten mice in each group: control group, hypoxic model group, monocrotaline injection model group(MCT group)and monocrotaline injection while low oxygen treatment model group.The mean pulmonary arterial pressure(m PAP), WT% and RVHI were measured.The expression of FHL1 and paxillin in the lungs of rats were measured by immunohistochemical staining.Results: The m PAP of hypoxic model group, MCT group and monocrotalinel injection while low oxygen treatment model group were significantly increased compared with the control group(P<0.01), those groups of RVHI were higher compared with the control group(P<0.05). WT% of the three groups were significantly higher than that the control group(P<0.01).The positive staining of FHL1 of the three groups(0.16±0.03,0.15±0.03,0.21±0.03) were significantly increased compared with the control group(0.08±0.02). the positive staining of paxillin of the three groups(0.19±0.03,0.21±0.04,0.22±0.04) were significantly higher than the control group(0.11±0.03).The expression of FHL1 and paxillin in the monocrotalinel injection while low oxygen treatment model group were increased compared with hypoxic model group or MCT group(P<0.05).Conclusion: FHL1 together with paxillin may play an important role in the pathogenesis of pulmonary vascular remodeling and pulmonary artery hypertension.Part II The Expression of FHL1 in the Serum of Patients withCOPD Induced pulmonary artery hypertension.Objective: To investigate the expression of serum FHL1 with COPD induced pulmonary artery hypertension and discuss the possible roles of FHL1 in the occurrence of pulmonary artery hypertension.Methods :The venous blood from the elbow of 40 patients with COPD induced pulmonary artery hypertension and 40 health patients were collected, The serum level of FHL1, Talin1, αVβ1 and Cyclin-D1 were measured by enzyme-linked immunosorbent assay(ELISA). Results: The optical density values of FHL1,Talin1,αVβ1 and Cyclin-D1 in patients with pulmonary hypertension group were 0.089 ± 0.029,0.113 ± 0.035,0.181 ± 0.054 and 0.031 ± 0.013,which were significantly lower than that of the normal control group: 0.133 ± 0.031,0.212 ± 0.044,0.251 ± 0.051 and 0.071 ± 0.026, and the difference between the two groups was statistically significant(P<0.01).Conclusion: The expression of FHL1 has a significant reduction in the patients with COPD induced pulmonary artery hypertension than that of the normal control group, this change indicates that FHL1 together with the other three factors may be associated with the formation of COPD induced pulmonary artery hypertension.