| Objective With the development of ancomy,the number of the open fracture accidents increased, the reason such as falling injuries,traffic accident increased,surgical procedures are not standardized is a main factor to causing bone infection caused by defects or chronic osteomyelitis.The infected bone defects is one of the most difficult problem in orthopedics, face of two difficult problems:one is the control of bone infect,the other is filling bone defecs, bone defect repair is have two methord one is autogenous bone,the other is Allogeneic bone substitute both of these can effective repair. Bone infect is most difficult,t Now patients are local injection of antibiotics to anti-infection treatment, sustained release topical antibiotic treatment of bone infection with a high concentration of systemic toxicity, etc., is considered to be an effective method for the treatment of bone infections.We choose for methicillin-resistant Staphylococcus aureus infection sensitive clinical ancomycin hydrochloride as a first-line antibiotic encapsulated drug.We use Controllable molecular biological degradation of polymeric materials PLGA and CS,Pre Pare the vaneomycin hydroehloride 一 loaded Poly laetic acid 一 glycolic acid Copolymer ancomycin by the multiple emuision methord,this matiteal have good Biocompatibility and biodegradable long-term release of antibiotics to control and cure of infectious material.Methods Pre Pare the vaneomyein hydroehlorid 一 loaded Poly laetic acidglycolic acid copolymer ancomycin by the multiple emulsion,by investigate PLGA /VA/CS the varity of factors,and evaluate the surface features and ancomyc properties,to filter the most important factor.,and use the orthogonal experiment fliter the best preparation.Microspheres were characterized by scanning electron microscopy(SEM)、UV、HPLC and other detection means to evaluate itsmorphology, particle size distribution, encapsulation efficiency, drugloaded,pulmonary retention, and accumulated release,Disk diffusion method to determine directly the pharmaceutically active suspension penthouse microspheres and antibacterial effect.Results Microspheres were characterized by scanning electron microscopy(SEM),UVHPLC and other detection means to evaluate itsmorphology, particle size distribution, encapsulation efficiencydrug-loaded,pulmonary retention,and accumulated release to determine the ancom.,Complete preparation of microspheres Sphere, smooth sphere, a small amount of folds, uniform particle size distribution in line with the normal distribution the average particle size(252.3±5.3) nm, the average drug loading was(3.12 ± 0.78)%, with an average encapsulation efficiency(33.57 ± 2.17)%.Under conditions of constant temperature 37 ° PLGA / VA / CS duplex sustained release microsphere time up to three months, with the disk diffusion, direct method to measure the drug active suspension method penthouse microspheres with antibacterial effect, double microspheres consistent with the activity of the VA VA standards, effective antimicrobial time up to 2 months.Conclusion Comprehensive survey of drug-loaded microspheres prepared by the prescription process,by duplex microspheres were characterized by particle size, in vitro release, antibacterial effects to achieve the desired goal.(1)Successfully established the method of UV spectrophotometry to measuring the PLGA content.(2)The effects factors of ncomycin hydrochloride sustained release microsphere preparation.(3)Optimize the vancomucin hydrochloride duplex microspheres formulation and technology.(4)Optimize the vancomucin hydrochloride duplex microspheres formulation and technology.,it have the andvantage of Burst, long slow release time, better repeatability and stability.(5)Investigated Duplex sustained release microsphere preparation process of PLGA/CS/VA, the most influence factor of In vitro drug release profile.(6)Investigated Optimize the formulation and technology.of PLGA/CS/VA sustained release duplex microspheres in vitro release effect. |
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