The Roles Of VE-Cadherin And P120 Catenin In The Formation Of The Experiment Intracranial Aneurysm In Rabbit

Objects:The formation of intracranial aneurysm(IA) was still unclear. In our previous studies, the phosphorylation of VE-Cadherin participated in the progress of formating IA, as well as the translocation of β-catenin into the nucleus of endothelial cells. As a regulating factor of the VE-Cadherin-Catenin compound, p120 catenin(p120ctn) played a critical role in regulating the function of endothelial cells. We designed this study to investigate the hypothesis that VE-Cadherin and p120 ctn participate the formation of IA, through building the model of experiment IA in rabbis, and testing the expression of VE-cadherin and p120 ctn in IA. Methods:20 New Zealand rabbits were selected and randomized into 2 groups(experiment group and control, 10 for each). In the experiment group, both carotid arteries of a rabbit were ligated to build the model of experiment IA in rabbits through the operation. In the control group, all rabbits underwent a same procedure as the experiment group, but carotid arteries were not ligated. The speed of blood flow in the basal artery was evaluated by through cranial doppler(TCD) at 1, 7,14, 28,48,56,70,84 days after the procedure accomplished. In the 12 th week after the operation, all rabbits were euthanized and the bifurcation of the basal artery were taken and analyzed through: 1, hematoxylin-eosin(HE) staining: morphological changes through the optic microscope. 2, immunofluorescense technique: the expression of VE-Cadherin and P120 ctn, the location of P120 ctn and its relationship with the core of endothelial cells, Results:1, the speed of blood flow in basal arteries. The speed of blood flow in basal artery increased significantly in the experiment group than that in the control group(p<0.05).2, morphological changes in the bifurcation of the basal arteries. Two types of morphological changes were detected in the experiment group: 1) the earlier stage of IA, which occured in 4 rabbits. Only the intima and median muscle of vessels were lightly injured. The wall of these basal arteries showed little morphological changed. 2) the forming of IA,which occured in the other 6 rabbits. The intima and median muscle of vessels were severe injured. The wall of vessels protruded outside as IA. No morphlogical changes were detected in rabbits of the control group.3, the expression of VE-cadherin and p120 ctn. In the control group, the VE-Cadherin and p120 ctn expressed linearly, uniformly and consecutively in the intima. In the experiment group, VE-cadherin and p120 ctn expressed inconsecutively in intima, and the fluorescenceintensity of VE-Cadherin and p120 ctn decreased significantly than that of the control group(p<0.05). The expression VE-Cadherin and p120 ctn in IA decreased significantly, compared with the early stage of IA. A part of p120 ctn translocated into the nucleus of endothelial cells. Conclusion:During the formation of IA, the expression of VE-cadherin and p120 ctn progressive decreased. p120 ctn translocated to the nucleus of endothelial cells as β-catenin, suggesting that p120 ctn, seperated from VE-Cadherin and translocated into the nucleus of endothelial cells, participated in the formation of IA.