The Study Of Effects Of L-asparaginase On Jurkat Cells’ Proliferation And Apoptosis

Objective : To study the effects of L-asparaginase on the proliferation and apoptosis of human acute T-cell leukemia Jurkat cells and approach the potential mechanism of action so as to provide experimental evidence for clinical applications.Method: Jurkat cells were cultured in vitro; different concentration L-asp interfered with Jurkat cells in logarithmic growth phase. The changes of jurkat cells that were interfered with different concentration L-Asp were observed by inverted phase contrast microscope. The morphological diversity was observed under a microscope with Gimsa stained cells. We extracted the cells’ total DNA of each group and then detected DNA fragments by agarose gel electrophoresis. Cells apoptotic rate and mortality that were interfered with different concentration L-Asp for 48 h were detected by Flow cytometry.Result:1. Jurkat cells morphology changed after treated with L-Asp. That included colony formation significantly reduced, cells mostly single suspended growth, cell volume sized, nuclear shrunken, nuclei deeply stained,chromatin dense lumped.2. DNA ladder can be showed by DNA electrophoresis after the jurkat cells were treated with 1IU/ml、2.5IU/ml、5IU/ml、10IU/ml L-Asp.3. The jurkat cells, treated with L-Asp in the concentration of 1IU/ml、2.5IU/ml、5IU/ml、10IU/ml for 48 h, can be induced apoptosis and death.The jurkat cells’ apoptosis rate was highest when treated with the concentration of L-Asp was 2.5IU/ml.The jurkat cells’ mortality rates increased with L-Asp concentration rising.Conclusion: The apoptosis of Jurkat cells can be induced by L-Asp.Apoptotic DNA fragmentation occurs. Apoptosis rate varies with concentration of L-Asp.The death rate increases with concentration of L-Asp. In short the concentration of L-Asp may determine its clinical action,but that whether it may induce resistance by apoptosis needs further study.