Damage Of Wistar Rats Liver After Using Hypo-fractionated Radiation And Oxaliplation

Objective: The purpose of our study is to clarify whether Hypo-fractionated radiation therapy combined with oxaliplatinon can aggravate liver damage, in order to provide a reference for our clinical application Methods: 80 Wistar rats were randomly divided into four groups: the control group(group A), the chemo-treatment group(group B), the radiation treatment group(group C), and the concurrent chemoradiotherapy group(group D). The liver tissue was collected for histological evaluation at the 1st, 2nd, 4th, 6th and 8th week after irradiation by HE staining. Meanwhile the expression of Bcl-2 and Bax was detected by immunohistochemical technique. Results: Histological examination showed swollen hepatocellular cells in the experimental group. Also, the degeneration and necrosis of liver tissue can be seen. Alanine aminotransferase(ALT) and Aspartate aminotransferase(AST) between the groups were significantly different at the same time,(F=85.869,214.663 P<0.001).Expressions of Bcl-2 and Bax were significantly different between each time point(F=6.047,43.344 P<0.05), and Bax were significantly different between each group(F=8.122 P<0.05),but Bcl-2 had no different between each group at the same time(F=0.808 P>0.05) Conclusion: Radio-chemotherapy may aggravate liver injury and it must be carefully used clinically. The overexpression of bcl-2 genes and the reduced expression of Bax genes may explain the results.