| Objective:To compare the difference between the ability to differentiate towards a nucleus pulposus-like phenotype induced by transforming growth factor-beta 1 of NP-MSCs and AD-MSCs. Methods:The groin fat tissue and the coccygeal spine of rats were taken respectively, NP-MSCs and AD-MSCs were sampled and cultured by mechanical enzyme digestion method. Flow cytometry instrument were employed to detect the expression of CD105, CD90, CD29, CD45, CD44, CD34, CD24 of both two kinds of stem cells. NP-MSCs and AD-MSCs were divided into complete induction group(complete induction medium), incomplete induction group(complete induction medium without TGF-β1) and the control group(DMEM/F12 containing 10% fetal bovine serum and100 mg/L penicillin/streptomycin) respectively. After 14 days of induced differentiation, RT-PCR were used to detect the expression of Collagen type Ⅱ,Aggrecan and SOX-9 of each group. Results:The expressions of CD105, CD90, CD29 were positive and CD45, CD44, CD34, CD24 were negative in both two kinds of stem cells. After 14 days of induced differentiation, the expression of Collagen type Ⅱ, Aggrecan and SOX-9 in complete induction group were significantly higher than that in the control group(P<0.05). The expression of Collagen type Ⅱ, Aggrecan and SOX-9 in NP-MSCs were significantly higher than that in AD-MSCs(P<0.05). Conclusion:These findings suggest that both two kinds of stem cells have the ability to differentiate towards a nucleus pulposus-like phenotype induced by TGF-β1, NP-MSCs may be more more suitable as seed cells for nucleus pulposus tissue engineering research. |
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