Expression Of The Changes Of Neuron Specific Enolase In The Model Of Oxygen Glucose Deprivation Injury Of Neuronal

Objective: To explore the relationship of the changes of Neuron specific enolase(NSE) with the model of oxygen glucose deprivation(OGD) of neuronal.Methods: To primary culturing hippocampal neurons of newborn(less than 24h) SD rat, evaluation of the neurons was carried out by fluorescence staining and electron microscope. To establish the hypoxic ischemic injury model of the rat hippocampal neurons by the application of oxygen glucose deprivation(oxygen-glucose deprivation, OGD), Extracting the proteins of neurons after reoxygenation in 1h, 6h, 12 h, 24 h, 48 h, and 72 h, Western-Blot method was used to detect the neuronal injury model at different time points in NSE level.Results: 1. A lot of neurons began to adhere to the.cover culture bottle aftern 4 to 6h. Increase over time, the neurons showed different shapes-triangle pyramidal, spindle and so on. Protuberant growth and coarsening, increase the cell body and full, they were different in length and thickness and connected to nets, form the neural network.2. The isolated and cultured cells were confirmed as neurons by fluorescence staining. It was clere for the nucleus and the form was typical to connect with nets.3. The NSE level began to increase in 6 hours, reached the peak in 24-48 hours, and began to decrease in 72 hours(P<0.01).Conclusions: 1. Establishing a serum-free primary culture method for cortical neurons of new-born rats. The neurons were purified by method of differential attachment. This technique is an easy and practical tool for primary culture of new-born rats cortical neurons with high purity, and can be used an in vitro model of research.2. Oxygen- sugar deprivation can cause damage to neurons, the neuronal specific enolase(NSE) of neuronal changed when a certain phase in different time points.