Font Size: a A A

Preparation And Characterization Of Nanoscale Cationic Lipid Microbubbles

Posted on:2016-04-07Degree:MasterType:Thesis
Country:ChinaCandidate:Y ZhangFull Text:PDF
GTID:2284330479982066Subject:Imaging and nuclear medicine
Abstract/Summary:PDF Full Text Request
Objective To prepare a nanoscale cationic lipid microbubbles and to investigate its characterization and ultrasonic imaging in vivo.Method Nanoscale cationic lipid microbubbles was prepared by filming-rehydration and machine vibration, with choosing DC-chol as cationic component of microbubbles. To observe difference in the appearance, distribution, diameter, zeta potential and contrast-enhanced ultrasound effect between homemade nanoscale cationic lipid microbubbles and Sono Vue.Results The homemade nanoscale cationic lipid microbubbles were dispersed uniformly and regular sphere. Its mean diameter and zeta potential was 442.6±58.75 nm and +(41.8±10.2)m V, and it had good stability by continuous observation within 1 week. In vivo enhancement effect strength of homemade microbubbles was slightly lower than Sono Vue, but its enhanced duration was longer.Conclusion The nanoscale cationic lipid microbubbles was a new gene transfection vector with a smaller particle size, a positive surface charge that can carry the gene and a good imagining effect in vivo characteristics, showing great potential for gene transfection carrier in vivo.
Keywords/Search Tags:nanoscale, cationic lipid microbubbles, ultrasound contrast agents
PDF Full Text Request
Related items