Acetylcholinesterase(AChE) Deficiency Protects Age Related Macular Degeneration Disease(AMD) From Cell Apoptosis

Objective To observe AChE deficiency or inhibition protects aged related macular degeneration disease(AMD) from RPE cell apoptosis.Methods ARPE19 cells were cultured in different concentrations of NaIO3. ARPE19 cells treated with NaIO3 were divided into four groups:control, 0.1mM, 1mM,10 Mm.The cell viability was detected by MTT Immunohistochemistry analysis was used to observe the expression of AChE.Then cells transfected with AChE siRNA and treated with AChE inhibitor(Donepezil)and AChE-/+mouse administrated sodium iodate(NaIO3)which concentration is 25mg/kg in tail vain.The apoptosis of ARPE19 cells were assayed by terminal transferase-mediated DNA end labelling assay,immunohistochemistry ananlysis and western blot analysis detected the expression of AChE and cleaved Caspase-3 and NOX2.Cell ROS detected the quantity of ROS.Results Compared with the control group,the cell viability reduced and AChE increased in the cells treated with Na IO3.After AChE deficiency or inhibition,the apoptotic cells decrease.And the expression of ROS and NOX2 were decrease,too.Conclutions AChE were expressed during NaIO3 induced ARPE19 cells apoptosis.AChE deficiency or inhibition through oxdative stress to decrease the apoptosis of RPE cells.