| Objective: By observing the astragalus glycosides of idiopathic pulmonary fibrosis(IPF) model in rats at different times general state, pathological histology changes, and basic fibroblast growth factor(BFGF) and microvascular density(MVD) expression in lung tissue, preliminarily astragalus armour glycosides of IPF prevention effect and influence on microvascular new mechanism.Methods: 130 SPF Wistar rats were randomly divided into 6 groups: blank group, model group, hormone, astragalus armour high, medium and low dose group, each group only 21. Using the trachea injection bo lai toxin(BLM) induced pulmonary fibrosis in rats model. Building the next day began to fill the stomach, continuous lavage treatment of 28 d, respectively in 7d, 14 d and 28 d partial to be put to death animals, collecting specimens. To observe the lung tissue of rats in general form, respectively by HE staining and Masson staining microscopic observation of rat lung tissue pathology morphological changes, using the method of SABC immunohistochemical techniques and combined with image analysis system, detection of BFGF lung tissue of rats and the expression of MVD.Results:1.General condition observed in ratsBlank group rats spirit and good diet, fur burnish, weight increase gradually, breathe smoothly. Model group rats eating less, listlessness, fur sparse, lacklustre, activity significantly fewer, shortness of breath, loss of weight. Hormone group of early and late diet decreased gradually, fur sparse, good quiet. High, middle dose group of early spirit not beautiful, color luster, breathing is not smooth, but later period spirit, diet and activity is good, fur more luster, smooth breathing, gradually increasing weight. Low dose group spirit, eating well, slow, weight gain is not obvious.2.The macroscopic observation of lung tissue in ratsNo obvious change in blank group rats lung tissue. Model group rats lung tissue marked hyperemia and edema, color dark red, rough surface, large petechiae, ecchymoses, uneven surface pale, the lesion was significantly heavier than the other groups. Compared with model group, radix astragali a nucleoside high, medium and low dose group and the hormone group lesion severity. Compared with hormone group, astragalus glycosides high, low and middle dose group of lesions significantly reduce. Astragalus armour glycosides close, low dose group and the hormone group compared with model group.3.HE staining and Masson observations.Blank group form has no obvious lung tissue lesions. Model group: 7d rat alveolar interval significantly broadening, inflammatory cells invasion, lung tissue structure shrinkage, collapse, exudate, 14 d alveolar inflammation, relieve, hyperplasia of fibroblasts and pulmonary fibrosis gradually appear, 28 d lesion is most the most severe fibrosis area. Compared with model group, compared to 7d, 14 d and 28 d high, medium and low dose group and the hormone group of alveolar inflammation and pulmonary degree of fiber decreased obviously. Compared with hormone group, 7d and 14 d and 28 d of high, middle dose group of alveolar and lower degree of pulmonary fibrosis. 7d,14 d and 28 d alveolitis and pulmonary fibrosis degree and low dose group close to the same hormone group.4.The Results of the microvascular density(MVD) expression14d, 28 d expression significantly higher, difference was statistically significant(P < 0.01). Compared with model group, radix astragali a nucleoside high, medium and low dose group, hormone MVD lung tissue in 7d, 14 d and 28 d expression is reduced, difference was statistically significant(P﹤0.05).Compared with hormone group, high, middle dose group of lung tissue MVD in 7d, 14 d and 28 d significantly reduce, difference have statistical significance(P﹤0.05). Compared with hormone group, low dose group of lung tissue MVD in 7d, 14 d and 28 d express no significance(P﹥0.05).5. The results of Basic fibroblast growth factor(BFGF) expressionCompared with the blank group, model group rats lung tissue BFGF expression in 7d, 14 d and 28 d expression enhanced obviously, the difference was statistically significant(P < 0.01). Compared with model group, radix astragali a nucleoside high, medium and low dose group and the hormone group BFGF expression in the expression of 7d, 14 d and 28 d, difference have statistical significance,(P﹤0.05). Compared with hormone group, high, middle dose group BFGF7 d, 14 d and 28 d obviously decrease, difference have statistical significance,(P﹤0.05). Low dose group of BFGF expression in 7d, 14 d and 28 d compared with the same hormone group, difference have statistical significance(P﹥0.05).Conclusion:1. Endotracheal intubation one-off injection bleomycin, copied IPF rat model2.Bleomycin is induced in the process of pulmonary fibrosis in rats, rat lung tissue pathologic histology changes alveolar inflammation in 7 days, 14 days, reduce 28 days alveolitis and pulmonary fibrosis.3.Bleomycin is drug induced pulmonary fibrosis in rats pathological process of microvascular disease, with 14 days is particularly active, with the increase of pulmonary fibrosis, microvascular new number gradually reduce.4.Bleomycin is induced in the process of pulmonary fibrosis in rats, BFGF is one of the important promoting angiogenesis factor, given astragalus armour after the intervention, BFGF expression decreased.5.Astragalus armour glycosides of high, middle dose group can relieve alveolitis and pulmonary fibrosis, thus inhibiting microvascular new Born, achieve the goal of prevention and treatment of IPF. Its role is associated with the suitable equivalent concentrations of drug. |
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