Study On Preparation And Reverse Drug Resistance Activity Of Psoralen-loaded Solid Lipid Nanoparticles

Psoralen has a strong reversal activity for MDR, but its poor water solubility leads to low bioavailability, this study was designed to prepare PSO-SLN and improve the reversal activity for MDR.PSO-SLN was prepared based on emulsification evaporation-low temperature solidification method, response surface methodology was used to generate an optimal formulation evaluated by encapsulation efficiency and particle size, the morphology, particle size, Zeta potential, in vitro release and other properties were investigated. Differential scanning calorimetry and infrared spectral were used to analyze the phase of PSO in PSO-SLN, the reversal activity of PSO-SLN were studied by MTT method.PSO-SLN possessed a rounded structure and uniform size, the optimal formulation was determined as follows: lipid was 300 mg, emulsifier concentration was 2.5% and PSO was 2.0mg, its particle size was 98.1 nm, EE was 84.1%, Zeta potential was-18.7 m V, in vitro release data indicated that PSO-SLN had apparent slow-release effect, infrared spectral and DSC data show that PSO might have formed a new crystal in SLN system. The results of MTT method proved that PSO-SLN can significantly improve the reversal activity compared with PSO.PSO-SLN prepared by emulsification evaporation-low temperature solidification method had better particle size and encapsulation efficiency, response surface experimental design can be used to optimize its prescription and PSO-SLN was able to improve the reverse activity of PSO.