The Study Of Effects Of Donor Age And SDF-1α On The Biological Functions Of Bone Marrow-derived Mesenchymal Stem Cells

Objective:This study aims at exploring 1) the proliferation and differentiation changes of rat bone marrow-derived mesenchymal stem cells(rBM-MSCs) in different age; 2) the influence of SDF-1α protein on the impacts of high-glucose(25mmol/L) to rBM-MSCs’ proliferation, apoptosis and migration. Methods:1. The rBM-MSCs in different groups were examined by Flow Cytometry.2. Neonatal rat cardiomyocytes were co-cultured with rBM-MSCs. The morphologic changes of rBM-MSCs and the proteins expression of troponin T were detected with immunofluorescence technique.3. The survival of rBM-MSCs in different groups was examined by MTT assay for proliferation and Hoechst 33258 staining for apoptosis.4. The migration of rBM-MSCs in different groups was assessed by transwell assay. Results:1. The percentage of rBM-MSCs in G0/G1 phase was increased with age advancing and the percentage of TnT proteins-positive rBM-MSCs was decreased with age advancing.2. High-glucose inhibits rBM-MSCs’ proliferation and migration while induces their apoptosis(p<0.05) when compared with low glucose, the specific CXCR4 receptor antagonist AMD3100 can further inhibit cell migration(p<0.05) but neither proliferation nor apoptosis(p>0.05), SDF-1α can attenuate such inhibitory effects(vs high-glucose, p<0.05). Conclusion:1. The ability of r BM-MSCs’ proliferation and differentiation descends with age advancing.2. SDF-1α protein attenuates the inhibitory effect of high-glucose on rBM-MSCs’ migration via CXCR4 receptors, while it may attenuate the inhibitory effect of high-glucose on rBM-MSCs’ survival via binding to non-CXCR4 receptors.