Preparation Of Gold Nanorods-porphyrin And Its Application In Tumor Thermal Technology

In recent years, nanomaterials are gradually applied to the diagnosis and treatment of cancer with the continuous development of nanotechnology. Gold nanorods is an excellent nanomaterials, which arise from their unique optical and photothermal properties, ability to tune the size and shape of the particles, the ability to modify the surface and conjugate drugs molecules to the nanorods, and the relative biocompatibility of gold nanorods, which makes it an excellent tumor photothermal therapy agent. However, tumor photothermal therapy by gold nanorods alone is lack of targeting. Porphyrin is a good cancer treatment photosensitizer and magnetic resonance contrast agents in medical imaging and has a very wide range of applications in the area of diagnosis and treatment of cancer. Numerous studies have shown that porphyrins can be preferentially concentrated in the tumor cells. Porphyrin itself has a good fluorescence effect, which is very beneficial to investigate the circumstances of phagocytosis of porphyrin drugs by observing the fluorescence intensity of porphyrins. In this paper, two advantages of photothermal therapy of gold nanorods and the aibility of porphyrin to selectively gather in tumor cells were well combined ogether, which firstly makes porphyrin thiolated, then combining gold nanorods with thiolated porphyrin through the formation of Au-S bond, and finally make GNRS- Porphyrin NPs covalently bond herceptin drugs together by the formation of an amide bond. GNRs-Porphyrin-Herceptin were characterized by UV–vis, fluorescence Spectroscopy and TEM, the results revealed that gold nanorods, porphyrin and herceptin were successfully bonded. The effect of GNRs-Porphyrin-Herceptin on cell was investigatived through a series of experiments, such as photothermal effect experiments, cytotoxicity assays, targeted phagocytosis experiments and photothermal killing ex periments. Photothermal effect experiments showed that the nanoparticles can convert light energy into heat energy effectively, killing cancer cells under near infrared laser irradiation with high thermal stability. Cytotoxicity assays indicated that GNRs-Porphyrin-Herceptin had a excellent biocompatibility and a very low toxicity to normal cell in the absence of near-infrared light irradiation. Targeted phagocytosis experiments and photothermal killing experiments of different tumor cells showed that GNRs-Porphyrin-Herceptin can be better uptaken by BT474 cells combined with MCF10 A cell and generated obvious therapy ablation effects to BT474 cells. which suggested that GNRs-Porphyrin-Herceptin have the ability to target and kill tumor cells by photothermal effect. We demonstrated that prepared GNRs-Porphyrin-Herceptin was photothermal conversion material of a good tumor targeting ability, and effectively combines the gold nanorods’ photothermal therapy and tumor targeting of porphyrins, coordinating cancer drug Herceptin, to achieve targeted, low toxicity and highly efficient killing tumor cells...