The Study Of The Anti CD14 Monoclonal Antibody On NF-kappa B(p65) Of Alveolar Aacrorphage In Rat With Acute Lung Injury

Objective:To explore the anti CD14 monoclonal antibody on NF- kappa B(p65) of alveolar macrorphage in rat with sepsis acute lung injury.Methods:15 male SD rats(150±20)g were randomly divided into 3 groups, group Sham, group CLP and group Anti-CD14.There were 5 rats in each group. The classic cecal ligation and puncture(CLP) method was used to make the animal model with acute lung injury. After the success of modeling 6h, we used ELISA to detect plasma MIP-2, IL-10 and Western blot was used to detect(p65) protein expression level left lung. Inferior lobe of right lung was taken for HE staining and the pathological changes of lung after section observation under light microscope. Sham group as control group. Statistical Methods SPSS13.0.Results:ELISA results showed that the plasma MIP-2 levels in group CLP was significantly higher than Sham group and Anti-CD14 group, plasma MIP-2 concentration in Anti-CD14 group was lower than CLP group. There were significant differences(P<0.05); comparison of plasma concentration of IL-10 3 group, that no statistical difference(P>0.05); Western blot results showed that CLP group and the Anti-CD14 group of NF-kappa B(p65) expression quantity is higher than Sham group(P<0.05), Anti-CD14 group of NF-kappa B(p65) expression was lower than CLP group(P< 0.05). HE staining showed CLP group and Anti-CD14 group lung appeared inflammatory cells, pulmonary interstitial thickening, hemorrhage, in Anti-CD14 group than in CLP group significantly reduced pulmonary manifestation.Conclusion:The anti CD14 monoclonal antibody could bind to receptors on the surface of alveolar macrophages of CD14 specific, prevent lung NF-kappa B(p65) nuclear translocation, reduce inflammatory cytokine production and release and inhibit inflammatory cell infiltration, plays an important role in the lung protective.