Insulin-like Growth Factor-1 Receptor Expression In Cells And Tissues Of Human Non-small Cell Lung Cancer

Objective:Influence of horizontal in vitro cell detection of IGF-1R monoclonal antibody on cell proliferation and invasion of non-small cell lung cancer; differences in immunohistochemical detection of IGF-1R levels in non-small cell lung cancer of different pathological types, different stages, different differentiation degree of expression, to provide a new method for early diagnosis of non-small cell lung cancer, according to IGF-1R pathway genes is demonstrated to the treatment of the theory and the practice foundation.Methods:This study is divided into two parts: human lung squamous cell carcinoma cells and human lung adenocarcinoma cells in vitro, and chemical observation and immunohistochemistry were identified under inverted microscope. Effect of MTT was detected in human lung squamous cell carcinoma cells and human lung adenocarcinoma cells, respectively by IGF-IR MAb with IGF monoclonal antibody-1R 100 μ g/ml, 10 μg/ml, 5 μ g/ml, 1 μ g/ml non small cell lung cancer cells for 48 hours, to detect the effects of IGF-1R MAb in human non small cell cancer cell proliferation and draw the curve.Detection of Transwell blank assay group, IGF-1R MAb group, cell migration and invasion,observe the effect of non plant invasiveness of small cell carcinoma cells, and draw the curve; 48 cases of lung squamous cell carcinoma, 35 cases of lung adenocarcinoma specimens, IGF-1R was detected by immunohistochemical method, IGF-1R in lung squamous cell carcinoma, lung adenocarcinoma in different stages of different degree of differentiation, expression of the difference in the expression of.Results:Human non-small cell carcinoma cells after 48 h incubation, cells lose the normal contact inhibition, a multilayer growth, growth metabolism, doubling time, saturation density, mitotic index higher, with infinite proliferation, cell invasion ability of expression;immune cells, IGF-IR in small cell lung cancer cell in the determination of the results show the non, non small cell lung cancer cell line IGF-IR is widely expressed in cell membrane and cytoplasm, mixed type expression, color was brown or brown; MTT assay showed that the IGF-IR MAb with 100 μ g/ml, 10 μ g/ml, 5 μ g/ml, 1 μ g/ml non small cell lung cancer cells 48 hours, inhibition by cell proliferation, inhibition rates were:(8.24 ± 1.02)%,(12.58± 0.94)%,(18.41 ± 1.15)%,(21.02 ± 0.87)%, with the increase of IGF-IR MAb concentration, the inhibition rate of cell proliferation increased gradually, comparison of different concentration groups between the two two groups, the difference was statistically significance(P<0.05); Transwell assay detection of blank control group, IGF-1R group MAb cell migration and invasion ability of IGF-1R group MAb, migration and invasion of Transwell cell membrane to the number of cancer cell surface was obviously Significantly lower than the control group(P<0.01). The specific expression of IGF-1R in lung squamous cell carcinoma, the expression of sensitivity and specificity were 57.1%, 97.8%,compared with the expression of IGF-1R in adenocarcinoma of the lung, the difference was statistically significant(P<0.05). IGF-1R protein expression in non-small cell lung cancer associated with gender, age, tumor size, tumor number, vascular invasion, TNM staging and other aspects of the difference was not statistically significant(P>0.05).Conclusion:Experimental verification of IGF-1R Mab cells in vitro objective non-small cell lung cancer cells inhibited cell proliferation and invasion. The high expression of IGF-1R protein in lung squamous cell carcinoma, and may be involved in the development of non small cell lung cancer, provide a new way for early diagnosis of squamous cell carcinoma of the lung.