Effect Of Nano Alumina Particles On The Regulation Of Immune System In ICR Mice

Objective By using different diameter of the nano alumina ICR mice after subchronic exposure,detection of different particle size of nano-alumina in the main organs of mice accumulate status, especially in the brain, spleen alumina accumulation situation, to reveal the distribution of nano-alumina particles enter in their mice after major organs; Detection of oxidative stress in mice main immuneorgans and corresponding cytokines to explore nano-alumina particles from the nasal cavity of mice after implantation immune system, revealing the different sizes of nano-alumina caused by the immune system in mice influence; The cytokines associated with the immune system were detected, to investigate the effect of nano alumina particles on the nervoussystem and immunesystem of mice after entering the mice, revealing the different sizes of nano-alumina regulating immunity of mice neural networks.Leading to the conclusion and to investigate particle size effect and dose nanoscale alumina particles of toxic effects in mice, in order to provide a strong basis and reference for the safety evaluation of nanoscale alumina particles.MethodsⅠ 、 3-month-old healthy SPF level 70 male and female ICR mice were divided into seven groups: control group, solvent control group, 13 nm particle size of Al2O3 low dose group(25mg / kg bw), 13 nm Al2O3 particle concentration group(50mg / kg bw), 13 nm particle size of Al2O3 high dose group(75mg / kg bw), 50 nm Al2O3particle size group(50mg / kg bw), micron size Al2O3 group(50mg / kg bw).Continuous thirty days three times daily intranasal exposure. Detecting the content of the spleen, heart, liver, lung, kidney aluminum element by the inductively coupled plasma emission spectrometer, and detecting brain tissue content of aluminum element by graphite furnace atomic absorption spectrometry.Ⅱ、3-month-old ICR mice of SPF 54, points 9 group, dose group were: control group,solvent control group, micron size aluminum group(50mg / kg bw), 13 nm diameter Al2O3 low concentrations(25mg / kg bw), 13 nm Al2O3 particle concentration group(50mg / kg bw), 13 nm diameter Al2O3 high dose group(75mg / kg bw), 50 nm particle size of Al2O3 low dose group(25mg / kg bw), 50 nm Al2O3 particle concentration group(50mg / kg bw), 50 nm particle size of Al2O3 high dose group(50mg / kg bw). 30 consecutive days, 25-35μl / day nasal drip.Enzyme-linked immunosorbent assay to detect ICR mice immune organs cytokine content;Determination of internal organs of mice immunized ICR superoxide dismutase of WST-1 method; TBA assay content ICR mice immune organs of MDA; DTNB cyclic reaction, the determination of ICR mice immune organs glutathione content.Ⅲ、3-month-old ICR mice of SPF 40, dose group were: control group, solvent control group, 13 nm Al2O3 particle size group(50mg / kg bw), 50 nm Al2O3 particle size group(50mg / kg bw), micron size aluminum group(50mg / kg bw). According to body weight of mice infusion 25-35μl / day, exposure month. Detecting ICR mice serum IL-1β, IL-4, IL-6, TNF-α levels by enzyme-linked immunosorbent assay.ResultⅠ 、 Compared with the solvent control group were high in nano-alumina content of aluminum in various organs, and the difference was statistically significant. Spleen,liver accumulation of its main organ, which 13 nm Al2O3 mainly accumulate in the spleen, 50 nm Al2O3 mainly accumulated in the liver; brain tissue 13 nm Al2O3exposure dose accumulated the largest amount of aluminum element, followed by50 nm Al2O3 exposure dose group.Ⅱ 、 After the nano-alumina particles into mice, the mice may produce inflammatory airway and lung irritation, release of inflammatory mediators and chemokines into the circulation system and cause oxidative damage and systemic inflammatory response and then start nonspecific immune function, cytokine network adjustment disorder, the immune system caused by different levels of oxidative damage,stimulate the immune system to produce an immune response; different diameter of the nano-alumina materials, the preliminary view that the impact 13 nm Nano-Al2O3 on the immune system caused by the maximum, 50 nm Nano-Al2O3 relatively weak,due to the impact of less than micron size alumina nano-grain alumina. Damage Index oxide nano-alumina particles in mice immune organs and cytokines in a dose- response relationship.Ⅲ、Nano-alumina different sizes of mice exposed to serum interleukin-1,6 and tumor necrosis factor-α excessive production and having a particle size-effect relationship,which makes inflammation worse, leading to neurological deficits symptoms,immune regulation harm; serum IL-4 content of each exposed groups have different degrees of reduction, the amount of IL-4 leukopenia will affect the autoimmune response mediated by Th1 cells.