The Expression Of ADAMTS-7 And ADAMTS-12 In The Degenearted Lumbar Intervertebral Disc And Its Clinical Significance

【Objective】To investigate the expression and the effect of ADAMTS-7 and ADAMTS-12 in the degenearted lumbar intervertebral disc Nucleus pulposus, To supply a new direction for researching the mechanism of the degenerative lumbar intervertebral disc.【Methods】The experiment group consisted of 40 cases of the fresh pulpy nucleus tissue which were obtained from patients with lumbar disc herniation by surgical intervention(22external anulus rupture, 18 external anulus intact), while 10 cases of the flesh pulpy nucleus tissue were harvested By operation for fracture of lumbar vertebrae acted as control group.The m RNA and protein expression of ADAMTS-7 and ADAMTS-12 in 50 specimens were detected by semi-quantitative RT-PCR and immunohistochemical staining. Then, the results were analysed statistically.【Results】The mean ADAMTS-7 and ADAMTS-12 protein and m RNA levels in the anulus fibrosus disrupted group and anulus fibrosus undisrupted group are both higher than the control group(p<0.01). Between the lumbar intervertebral disc of experiment group, the expression of the ADAMTS-7 and ADAMTS-12 in the anulus fibrosus disrupted group is higher than that in anulus fibrosus undisrupted group(p<0.01).【Conclusions】ADAMTS-7 and ADAMTS-12 in the cells of human lumbar intervertebral disc may be involved in the progress of human lumbar intervertebral disc degeneration,this reseachhas certain clinical significance and with the increase of degree of human organization of lumbar disc degeneration, the expression of ADAMTS – 7 and ADAMTS- 12 gradually rise.