The Significance Of Diagnosis Of WT1 And NKX6-1 Gene Methylation In Cervical Cancer In Xinjiang Uigur Of Women

Objective:To explore the promoter methylation of WT1 and NKX6-1 genes in cervical cancer of Xinjiang Uigur of women. To analyze the relationship between gene methylation status and infection of HPV16,18,and study on the gene methylation influence on the gene of m RNA expression. Analysis correlation between the two gene promoter methylation and cervical cancer of Xinjiang Uigur of women and study on the feasibility of early diagnosis in cervical cancer.Methods:1.Collected 43 cases of normal cervical tissues, 30 cases of cervical intraepithelial neoplasia(CIN) tissues and 48 cases of cervical cancer tissues in Xinjiang Uigur of women. 2.PCR method was used to detect infection status of HPV16 and HPV18. 3.Methylation specific PCR method was used to detect the promoter methylation of WT1 and NKX6-1 genes, and analyze the relationship between the gene promoter methylation and HPV16/18 infection. 4.Select the β-actin as internal control, real-time quantitative reverse transcription- polymerase chain reaction(q RT-PCR) technique used detecting m RNA expression levels of the two genes. And analysis of the gene promoter methylation affect on the genes of m RNA expression. 5. Data analysis was carried out using statistical package SPSS 17.0, specificities significance was set at P<0.05. Using infection of HPV16/18 and gene methylation calculate the diagnosis of sensitivity and specificity in cervical lesions.Results:1. WT1 gene promoter methylation rate in the normal cervical tissues, CIN and cervical cancer group were 6.98%, 36.67% and 89.58%, respectively, three groups were statistically significant differences(P <0.01); NKX6-1 gene methylation rate in three groups were 11.63%, 46.67% and 77.08%, respectively,the differences were statistically significant(P <0.01). 2. HPV16 and HPV18 infection rate in the normal cervical tissues, CIN and cervical cancer tissues were 13.95%, 36.67% and 68.75%, the difference were statistically significant(P <0.01). 3. WT1 gene methylation in 68 cases of CIN2, CIN3 and cervical cancer tissues were related to HPV16/18 infection(P <0.05), whereas NKX6-1 gene methylation did not display correlation(P>0.05) 4. WT1 gene of promoter methylation of 10 cervical tissues m RNA expression level was significantly lower than 10 cases of the gene promoter non-methylation of the cervical tissues(P<0.01). NKX6-1 gene of promoter methylation of 10 cervical tissues m RNA expression level was significantly lower than 10 cases of the gene promoter non-methylation o tissues(P <0.01). 5. WT1 and NKX6-1 genes methylation for the diagnosis of cervical lesions of sensitivity and specificity were 79.4%,94.3% and 73.5%, 88.7%, respectively. They were both higher than the diagnosis efficacy of HPV16/18 infection of 61.8%,84.9%.Conclusion : The promoter of WT1 and NKX6-1 genes methylation were related to cervical cancer tumorigenesis in Uigur of women in Xinjiang. HPV16/18 infection may result in the promoter of WT1 gene methylation. Furthermore, the promoter of WT1 and NKX6-1 genes methylation is possible as a biomarker for diagnosis of cervical cancer.