Study Of The Relationship Between Diamine Oxidase And Intestinal Fatty Acid Binding Protein And Gastrointestinal Dysfunction In Asphyxia Neonates

Objective:The purpose of the study is to detect the two amine oxidase(DAO) and intestinal fatty acid bind ing protein(I-FABP) level in plasma of the neonates with asphyxia after birth at the first day and fifth day, as well as the relativity between gastrointestinal dysfunction score and the neonates with asphyxia, so as to explore the relationship of gastrointestinal dysfunction of asphyxial neonates with DAO and I-FABP, and determine the relationship of different degrees of asphyxia with gastrointestinal dysfunction of neonates, which would strengthen the understanding of neonatal asphyxia clinically, guide the clinical diagnosis and select the appropriate treatment program and improve the rate of successful treatment and long-term prognosis of asphyxia neonates.Methods: 60 cases of asphyxia neonates enrolled in the neonatal intensive care unit(NICU) of the first affiliated hospital of Shihezi University were divided into mild asphyxia group 40 cases and severe asphyxia group 20 cases according to the diagnostic criteria of neonatal asphyxia of "practical neonatology". We took the DAO and I-FABP ELISA kit of ROCHE company of Geman to detect DAO and I-FABP of the asphyxial neonates at the first day and fifth day after birth and the same parameters were detected in the control group.Results: 1.Comparison of DAO and I-FABP between different asphyxia group and the control group at the first day and fifth day after birth: DAO of the first day: The DAO of mild, severe asphyxia group and control group were(10.14 + 2.98) u/ml,(15.54 + 2.81) u/ml and(6.78 + 0.67) u/ml respectively. I-FABP of the first day: The I-FABP of mild, severe asphyxia group and control group were(149.1 + 12.04) u g/l,(325.8 + 20.13) ug/l and(50.30 + 7.56) ug/l respectively. The DAO and I-FABP of mild and severe asphyxia group were higher than that of the control group significantly(p<0.05). The DAO and I-FABP of severe asphyxia group was higher than that of mild asphyxia group. DAO of the fifth day: The DAO of mild, severe asphyxia group and control group were(6.80±0.70)u/ml、(10.69±3.08)u/ml and(6.99±0.89)u/ml respectively. I-FABP of the fifth day: The I-FABP of mild, severe asphyxia group and control group were(50.27±8.59)ug/l、(126.0±24.36)ug/l和(49.87±7.42)ug/l respectively. So the DAO and I-FABP reduced gradually as time went on, and they recovered to normal basically in mild asphyxia group. There was no statistical difference between the mild asphyxia group and the control group. The level of DAO and I-FABP of severe asphyxia group were still higher than that of the mild and control group. 2. The analysis of DAO and I-FABP in the asphyxial neonates with or without gastrointestinal dysfunction: The DAO and I-FABP in the asphyxial neonates with gastrointestinal dysfunction were(7.88±2.41)u/ml and(156.49±34.08)ug/l respectively and they were(7.88±2.41)u/ml and(156.49±34.08)ug/l respectively in the asphyxial neonates without gastrointestinal dysfunction. So the DAO and I-FABP in the asphyxial neonates with gastrointestinal dysfunction were significantly higher than that of asphyxial neonates without gastrointestinal dysfunction(p<0.05). 3. There were 22 cases with gastrointestinal dysfunction in the mild asphyxial neonates. The mild gastrointestinal dysfunction were 18 cases and the severe were 4 cases. In thesevere asphyxial neonates, there were 17 cases with gastrointestinal dysfunction. The mild gastrointestinal dysfunction were 7 cases and the severe were 10 cases.Conclusions: The gastrointestinal function of asphyxial neonates was related to the severity of asphyxia. The levels of DAO and I-FABP in the plasma were positively associated with gastrointestinal dysfunction and they were increased at the early phase of asphyxia and decreased gradually with the recovery of disease. The severer gastrointestinal dysfunction and asphyxial injury, the higher levels of DAO and I-FABP would be. They had good correlation, which could help to estimate the severity of asphyxia of neonates and guid treatment.