Effects Of TCF3 On Cell Proliferation And Migration In Non-Small Cell Cancer A549 Cells

Objective:To investigate the effect of T cell factor 3 (TCF3) gene silencing on the Wnt pathway transcriptional activity and cell growth and migration in non-small cell cancer A549 cells.Methods:The TCF3 gene was silenced by RNAi technology and used as the experimental group, meanwhile the blank control group and negative control group were set up.The mRNA and protein expression levels were detected by real-time fluorogenic quantitative PCR and Western blot separately.A TOPflash/FOPflash repoter gene was employed for analysis of the Wnt pathway transcriptional activity. The cell proliferation was detected by CCK-8 assay.The apoptosis and cell cycle were analyzed by flow cytometry.Transwell chamber assay was used to examine the migration potential of A549 cells.Results:Compared with those of blank and negative control groups, (0.17±0.02VS1.00±0.00, P=0.00; 0.17±0.02VS 1.00±0.17, P=0.00) and protein (0.03±0.01 VS0.15±0.02, P=0.00; 0.03±0.01 VS 0.15＋0.02, P=0.00) levels of TCF3 were lower in the experimental group.The ratio of TOPflash/FOPflash was lower (5.20±0.60 VS 10.30±0.90, P=0.00; 5.20±0.60 VS 10.70±0.40,p=0.00).The protein expression of Wnt target C-myc was declined (0.27±0.02 VS 0.75±0.04, P=0.00; 0.27±0.02 VS 0.74±0.04, P=0.00).The cell proliferation was inhibited. The apoptosis was promoted (29.77±1.52 VS 14.59±1.99, P=0.00; 29.77±1.52 VS 15.33±1.97, P=0.00) and the percentage of A549 cells in G0/G1 phase was increased (71.98±0.51 VS 59.20±2.55, P=0.01; 71.98±0.51 VS 58.06±3.25, P=0.00) and that in S phase was decreased (23.91±0.05 VS 30.87±1.80, P=0.00; 23.91±0.05 VS 32.87±3.90, P=0.01).The cell migration ability was reduced (36.00±4.00 VS 68.70±4.20, P=0.00 ±, 36.00±4.00VS67.00±8.70, P=0.00)Conclusion:1.Knockdown of TCF3 in A549 cells reduces the Wnt pathway transcriptional activity and down-regulates the expression of Wnt target C-myc, which demonstrates that TCF3 may plays a role of activating transcription。 2.TCF3 may affects the growth and migration of lung cancer cells by the regulation of Wnt target genes.