Focused Ultrasound Targeted Microbubble Destruction Supporting The Chemotherapy In H22 Liver Tumor

Objective:To study the ultrasound-targeted microbubble destruction (UTMD) is able to increase doxorubicin (DOX) drug concentration to supporting the chemotherapy in the mouse H22 liver tumor.Methods:We have built H22 liver tumor model in this study.120 mice were randomly divided into A and B groups. Each group include DOX injection group, UTMD group, DOX injection+ultrasonic irradiation group, DOX injection+UTMD group, control group and normal mouse is CB group. We start intervention at the 6th day after inoculation every two days. Group A:mice were sacrificed at 8,12,16,20,24th day, tumor growth was observed and the doxorubicin concentration within the tumor were determined. Microvessel density were tested by CD31IHC staining.Group B:To observe the blood flow signals of tumor in the liver when UTMD happened and we analyzed the survival rate in each group.Results:UTMD could enhance blood flow signals inside the tumor. The doxorubicin concentration in the tumor and the tumor inhibition rate was the highest in DOX injection+UTMD group while tumor volume, weight and MVD were the lowest at DOX injection+UTMD group.Conclusion:UTMD could transiently increase the internal tumor blood flow and improve the DOX concentration. The tumor growth rate were restrained, This method is expected to become a novel chemotherapy strategy in the future.