Inhibitory Effect Of Suramin On Inflammatory Response In Pulmonary Tissue And Peripheral Blood In Septic Mice

Objective:Sepsis is that the interaction between environmental factors and genetic factors polygenic diseases, the lung as sepsis in the pathological process one of the most easily affected target organ, its research value is widely concerned. Suramin has a broad spectrum of biological effect, especially plays an important immune regulation in anti-inflammatory and antiapoptotic by influencing the NF-kappa B and TNF-alpha cytokines and so on. Therefore, to investigate whether suramin could inhibit the inflammatory response either in pulmonary tissue or in peripheral blood in septic mice, forther to explore the possible mechanism.Methods:24 male C57BL/6 mice were randomly divided into saline group and suramin group. Saline or suramin (5mg/kg) was injected in vein 30 min before LPS (5mg/kg) infusion.The expression of TNF-a and IL-6 protein levels in the pulmonary tissue and peripheral blood were detected by ELISA. In vitro, THP-1 cells were stimulated by LPS (100ng/ml) with saline or suramin pre-treatment. The activity of TNF-a and IL-6 was analyzed by PCR at 60 min after LPS insult, the activity of NF-κB were analyzed by western blot at 30 min、60 min and 90 min after LPS insult.Results:Compared with the saline group, the expression of TNF-a and IL-6 in the pulmonary tissue and peripheral blood were obviously reduced by suramin treatment at 24 h after LPS injury (P<0.01 or P<0.001). While there was no difference in those cytokines expression levels at 72 h (P>0.05). The activity of NF-κB was highly down-regulated by suramin at 30 min、60 min and 90 min after LPS insult.Conclusion:Suramin protected LPS-induced acute lung injury through down-regulated the expression of systemic and pulmonary pro-inflammatory factors, which was possible due to the inhibition of the NF-κB activity..