| objective:Periodontal ligament (PDL)tissue is a connective tissue that is interposed between the roots of the teeth and the inner wall of the tooth-supporting bone (alveolar bone) socket. The collagenous fibers form a meshwork that stretches out between the cementum covering the root surface and the bone and is firmly anchored by Sharpey fibers. The periodontal ligament links the teeth to the alveolar bone proper, providing support, protection, and sensory input to the masticatory system. In addition, the PDL also contributes to tooth nutrition, homeostasis, and the repair of damaged periodontal tissue. PDL cells originate in part from the ectomesenchyme of the investing layer of the dental follicle; this developmental origin gives these cells differential properties. Recently it has been revealed that PDL tissue possesses multipotential mesenchymal stem cells that can differentiate into mineralized tissue-forming cells such as osteoblasts and cementoblasts.Periodontitis is a chronic destructive disease, can cause periodontal support tissue damage, resulting in loss of teeth. The ultimate goal of periodontal therapy is completely recover lost periodontal attachment, caused by trauma or periodontal disease including cementum, periodontal membrane, alveolar bone restoration, therefore in the treatment of periodontal disease is a complex system engineering. Growth factor is the target the outside of the cell membrane receptor, signal and promote epithelial and mesenchymal cell migration, differentiation, increase matrix synthesis, but also can promote the regeneration of periodontal new attachment formation and periodontal tissue, phenotypic differentiation of periodontal ligament cells have a certain role. Periodontal tissue regeneration and reconstruction is a variety of cells participate in the molecular regulation, signal process. Although the study of oral tissue engineering has made great progress, but in the dental and periodontal tissues regeneration study, is still faced with great difficulties and challenges. If the repair process of clear body of the periodontal tissue that is how in the multiple factors, so many control completion, various cell growth factor synergy mechanism, and how the compatibility between them, collocation, optimal dose, so that the treatment of periodontal disease provides a new or treatment options effective, patients with periodontal disease can be exempted from the tooth. In view of this, this paper through the detection of PLAP-1, rhBMP-2, effect of rhBMP-4 on the proliferation of periodontal ligament cells, and the establishment of animal experimental model, exploring new treatments to periodontal tissue regeneration and reconstruction, so as to achieve safe and effective in the treatment of periodontal disease objective. Methods:by technique of cell culture in vitro, with thiazolium salts (MTT) method for the detection of different concentrations of PLAP-1 and rhBMP-2, rhBMP-4 alone and combined effects of human periodontal ligament cells proliferation, OD was measured with enzyme mark instrument, choose the best concentration of cell proliferation and culture time, cell proliferation was detected by enzyme kinetic method alkaline phosphatase activity was concentration and time. This result to establish animal model, PLAP-1 and rhBMP-2, the interaction effect between rhBMP-4 three of new cementum, alveolar bone height and the height of the new connective tissue attachment. Results:Compared with the control group, the PLAP-1 concentration of 50ug/l was the best concentration, concentration of 100ug/l rhBMP-2/rhBMP-4 was the best concentration. PLAP-1, rhBMP-2 and rhBMP-4 used alone can fibroblast ALP activity increased periodontal membrane, but the three combined effects, failed to enhance periodontal ligament fibroblast ALP activity, the difference was statistically significant, P<0.05.Conclusion:PLAP-1 in the differentiation and mineralization in periodontal ligament cell negative regulation may be achieved by adjusting the rhBMP-2, rhBMP-4, thereby preventing periodontal tissue non-physiological mineralization such as ankylosis. |
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