Property Of Anti-TRAIL Monoclonal Antibody And Identification Of The Epitopes

TRAIL (TNF-related apoptosis-induced ligand) is a new member of TNF superfamily.It contains 281 amino acid residues, and the activity site of TRAIL is the 114-281 amino acid residues which is the extracellular part of this protein (sTRAIL). Similar to other cytokine ligands of TNF superfamily, TRAIL shows a strong capacity to induce tumor cells apoptosis. Moreover, TRAIL could induce most transformed cells apoptosis without inducing most normal cell apoptosis. Thus, TRAIL attracts more and more attentions for cancer therapy. In these research processes of TRAIL, the monoclonal antibody is necessary.In this article, we characterize two anti-TRAILmAbs, named Abland Ab2. Ig subclass and specificity of mAbs were analyzed by ELISA and SDS-PAGE, respectively. The titres of mAbs in ascetic fluid were determined by indirect ELISA. Flow cytometry and MTT test were utilized to analyze the apoptosis-neutralizing activity. The apoptotic function of TRAIL was neutralized significantly by these two anti-TRAIL mAbs detected by flow cytometry and MTT. At last, we determined the epitopes of Abl and Ab2 by Phage display.