Effects Of Different Processed Terminalia Products On Protective Effects On Acute Liver Injury And Intestinal Propulsion In Mice

Objective:Acute liver injury models were constructed of different types of pathological to evaluate the different processed products of Terminalia which were in mice induced by D-GalN and CC14. Secondly to explore different excited states in the small intestine of mice intestinal affect in promoting different processed products, and provide a theoretical basis for the Tibetan Terminalia further optimization of the process and clinic applicationMethod:1. Evaluation of different processed Terminalia products of liver protective effects were by the establishment of acute liver injury models.1.1 The use of CCl4 maked acute liver injury in mice. The Kunming mice of SPF 120, male and female, were randomly divided into 12 groups, orally for 7 days, after the last administration 30 min, in addition to the control group of mice by intraperitoneal injection of saline 0.1 ml/lOg by weight, the rest of groups were according to body weight by intraperitoneal injection of 0.010mol/L CCl4 oil solution of 0.1 ml/10g body weight. After 18 h post-orbital blood sampling,tesded the serum ALT, AST, determination of GSH-Px in liver homogenates, observed HE staining of liver biopsy for reading pathological changes. In order to evaluate the protective effects of Terminalia different processed products made of CCL4 acute liver injury.1.2 Using D-GalN induced acute liver injury in mice. The Kunming mice of SPF 120, male and female, were randomly divided into 12 groups. Gavage for 7 consecutive days, after the last administration 30 min, in addition to the control group of mice by intraperitoneal injection of saline 0.1 ml/10g body weight, the rest of groups according to body weight were injected D-GlaN 800 mg/kg, After 24h post-orbital blood sampling, testing the serum ALT, AST, determination of GSH-Px in liver homogenates, observed HE staining of liver biopsy for reading pathological changes. In order to evaluate the protective effects of Terminalia different processed products of D-GalN acute liver injury.2. Effects of different processed Terminalia products were on different conditions, which were the intestinal propulsion rate. In three states, The Kunming mice of SPF 110, male and female, were randomly divided into 11 groups. Gavage, continuous administration of 3 d, before the last administration of water fasted 12 h, in addition to the control group after dosing interval 30 min, to give other measurement of physiological saline, other groups corresponding injection molding drugs, interval 15 min drugs given carbon ink per 0.2 ml. except normal state, after giving the inhibitory state indicator 20 min, the mice died of cervical dislocation; hyperactive state after 15 min, mice died of cervical dislocation. Calculated ink in the small intestine propulsion percentage.Result:1.Acute liver injury pathological models were duplicated in mice induced by hepatic D-GalN and CCl4. Different processed Terminalia products were intragastric prophylaxis,which could reduce acute liver injury by CCl4 or D-GalN which caused serum ALT, AST activity increased (P<0.05), liver tissue GSH-Px reduced was not significant, and reduced the acute liver injury pathological damage effectively. Madder Terminalia ig high dose group can significantly reduce CCl4 or D-GalN pathological model of acute liver injury induced by serum ALT, AST levels in liver homogenates GSH-Px activity and reduce the model group was not significant (P>0.05) were observed by HE staining of liver tissue, Madder Terminalia high dose group and the control group differences small changes in liver pathology.2. Different processed Terminalia products, for the gut in the normal state, the original drug Terminalia high, medium and low dose three groups; madder Terminalia high, medium and low dose groups and the control group is not significant (P>0.05), the original high-dose Terminalia, Madder Terminalia Terminalia high-dose and high-dose chamaejasme three groups and neostigmine groups was not significant (P>0.05), the other groups were significantly (P<0.05); the gut on excitation and inhibition states, the original Terminalia high, medium and low dose groups, Madder Terminalia high, medium and low-dose groups and Chamaejasme Terminalia high and low dose comparison with the control group were significantly, with the model group was not significant (P>0.05).The middle dose group of Euphorbia fischeriana Steud per Terminalia chebula Retz. was in inhibiting state, which compred with the model group was significant and with the control group was not significant (P>0.05). The middle dose group of Euphorbia fischeriana Steud per Terminalia chebula Retz. was in hyperactive state, with the model group which was not significant (P>0.05), with the control group which was significantly. (P<0.05)Conclusions:1. Special processing Terminalia of efficacy may change. Through experiments, the original drug and madder Terminalia for acute liver injury in preventing effects on acute liver injury in mice induced by CCl4 or D-GalN have evidenced. Special concocted Terminalia will be used to treat liver injury, which has medical significance.2. Effects of Special concocted Terminalia for intestinal propulsion rate, which the original drug and madder Terminalia were not significant. Chamaejasme myrobalan added Chamaejasme which have a role for intestinal propulsion. The period when the drug in the gut residence time is shortened, is relatively shorter time for the drug absorption, which is for drug administration interval.