Study On Killing Effects Of Cytokine Induced Killler Cells On Lung Adenocarcinoma And Ovarian Cancer Cell Lines

Background:Malignant tumor is one of the major threats to human health. The pathogenesis of lung cancer is concealed. Most patients are already in advanced stage when they are diagnosed. The incidence of ovarian cancer accounting for 20% of gynecologic malignanttumors has a tendency to rise year by year. The main methods of malignant tumor treatment are surgery, radiotherapy and chemotherapy. Tumor curative effect is severely limited by radiation resistance and multiple drug tolerance. Therefore, we urgently need to find the methods of more effective treatments. Adoptive cellular immunotherapy is a new kind of malignant tumor treatment technology, which makes up the defect of the traditional means of anti-tumor. It is widely used due to its little side effect, range of application and well curative effect, which becomes the powerful supplement of traditional cancer treatments. Cytokine-induced killer(CIK)cell is one of immune cells which are most widely used to clinic. CIK cells are a heterogeneous subset of immune cells generated by the multiple cytokines, and characterized by its dramatic powerful proliferation capability, cytotoxic activity, non-MHC-restriction and few side effects, which draw an increasing attention since its discovery. At present, CIK cells have been used in the treatment of malignant tumors and become a promising approach of comprehensive treatment of cancer.Objective:To investigate the killing effect of CIK on lung adenocarcinoma cells(A549), lung adenocar-cinoma’s radiation resistant cells(A549RR),ovarian cancer cells(SKOV3) and its drug resistant cells(SKVCR) in vitro.Methods:Peripheral blood mononuclear cells(PBMC) of healthy volunteers were stimulated by different cytokines, and induced into killer activity CIK cells. The phenotypes of CIK cells were analyzed by flow cytometer.A549, A549 RR, SKOV3 and SKVCR cells were cultured separately with the CIK cells. The optical density(OD)of the cells was measured by CCK8. And the killing rates were calculated in all group cells which were cultured for 24 and 48 hours with CIK.Results:1. The proportion of CD3+ CD56+ cells was 45.8%, when peripheral blood mononuclear cells(PBMC) of healthy volunteers stimulated by different cytokines were cultivated for 14 days in vitro.2. The killing effect was improved with the effector-target ratio increasing from 5 : 1 to 40 : 1, and it was also stronger when the cultured time was prolonged to 48 h compared to cultivated to 24 h(P < 0.05).3. The killing effect of CIK on A549 and A549 RR cells had no significant difference in the same culturing time and the same ratio of effective cells to the target cells(P > 0.05). The killing effect of CIK on SKOV3 and SKVCR cells had no significant difference either(P > 0.05).Concusion:CIK cells have strong anti-tumor effect against lung adenocarcinoma and ovarian cancer cells. The effector-target ratio and cultured time are the related factors of killing effect. The killing effect of CIK is similar between lung adenocarcinoma parent and radiation resistant cells, ovarian parent and its multiple drug-resistant cells, which has strong potential clinical application value.