XAV939 Inhibition Of Vasculogenic Mimicry Formation In Human Hepatoma HepG2 Cells

Objective:To investigate the impact of XAV939 to human hepatoma HepG2 cells vascular mimicry and its possible mechanism.Methods:HepG2 cells were divided into control group and XAV939 treatment groups with dose of 0.5 and 1μmol/L; Tube formation of tumor cells experiment to detect the forming tubes ability of each group, RT-PCR were used to detect expressed ZEB1 and MMP-7 mRNA levels, Western blot were used to detect expression of (3-catenin, ZEB1 and MMP-7 on protein levels.Results:The number of vascular mimicry in the control group and the experimental group. In the control group:9.67±0.70,0.5μmol/L group: 5.67±0.64, 1μmol/L group:2.27±0.81,which means the number of pipeline structure formation reducing in vitro (P<0.01). Experimental group compared with the control group, ZEB1 and MMP-7 mRNA expression reduce; ZEB1, MMP-7 and β-catenin protein expression decreased(P<0.05).Conclusion:XAV939 can effectively inhibition of vasculogenic mimicry formation in human hepatoma HepG2 cells.