Effects Of Sirna-Mediated Nestin Silencing On Invasion And Metastasis Of Human Esophageal Cancer ECA109 Cells And The Possible Mechannism

Objective:To investigate the effect of small interference RNA-mediated silencing of the nestin gene on cell invasion and migration in human esophageal cancer ECA109 cells and the possible mechanism.Methods:The esophageal cell line ECA109 was transfected with a small interfering RNA (siRNA) targeting nestin and the cell invasion and migration changes were observed. Real-time PCR and Western blotting were used to examine the changes in the expressions of nestin, MMP2, MMP9, VEGF andβ-catenin (total and nucleus) proteins in the transfected cells.Results:The As compared with the control group, the expression of nestin mRNA and protein was significantly down-regulated in ECA109 cells transfected with siRNA targeting nestin; So was expression levels of MMP2, MMP9 VEGF and(3-catenin (total and nucleus) proteins; The levels of invasion and migration capacity of ECA109 cells transfection with Nestin-siRNA had lower than the cells in the transfection with contorl-siRNA group.Conclusion:Knockdown GRP94 expression significantly inhibit the level of lung carcinoma A549 cells proliferation, which may act via influencing the expression of c-myc, cyclinD1 Knockdown nestin expression significantly inhibit the level of esophageal cancer cells invasion and migration, which may act via suppressing β-catenin translocates to the nucleus and influencing the expression of MMP2, MMP9 and VEGF.