| Objective To observe the growth characteristics of human umbilical cord mesenchymal stem cells (hUC-MSCs) cultured in alginate gel scaffolds and explore the feasibility for wound dressing model of hUC-MSCs-alginate complex.Methods hUC-MSCs were separated and identified in vitro. Then cells were cultured in 150mM calcium alginate, the proliferation and migration capacity, and expression of vascular endothelial growth factor (VEGF) of hUC-MSCs was investigated in the three-dimensional (3D) gel system. Subsequently, complex of alginate with hUC-MSCs was covered on the excisional full-thickness skin murine models. Then the skin wound healing and histological characteristics of the new skin were evaluated.Results hUC-MSCs grew well with grape-like proliferation in 3D alginate gel, but they could not migrate from the gel after 7days cultivation. ELISA showed that the expression of VEGF in the 3D group was significantly less than in the control group in first 3 days, while it was significantly larger in the 6th and 9th days (p<0.05). Besides, hUC-MSCs-alginate gel complex could significantly improve skin wound healing compared to control group.Conclusion hUC-MSCs can continue expressing massive VEGF that is indispensable for wound healing in alginate gel, hUC-MSCs-alginate gel compound probably be a good wound dressing. |
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