The Experimental Study Of Phase-Change Ultrasound Molecular Probe Physical Destructing Hepatocellular Carcinoma Cell

PARTⅠ STUDY ON PREPARATION AND PHASE TRANSITION OF LIQUID-GAS CHANGING ULTRASOUND MOLECULAR PROBE SULFATE CEREBROSIDES MODIFIEDObjective To prepare a novel nano ultrasound molecular probe-sulfate cerebrosides modifying lipid nanoparticles coating with Perflenapent,and to study SL-NP’s phase transition induced by thermal and ultrasound.Methods SL-NP was prepared by using film dispersion method,the physics features such as morphology、size、distribution were observed by optical microscope;the size and were detected by laser particle analyzer; with heating board heating, liquid-gas transtion of SL-NP was observed by optical microscope,after irradiation of low-intensity focused ultrasound(LIFU), acoustic droplet vaporation (ADV) of SL-NP was observed by ultrasonic diagonstic apparatus.Results The appearance of the prepared SL-NP was opacified suspension,spherical,high density,uniform size,uniform dispersion and without accumulation,the mean of size was (182.7±63.8)nm;when the temperature of heating board rised to 44.5 ℃, SL-NP began to arise liquid-gas transtion,with the temperature rising,the volume of phase-change nanoparticle continued to increases,until rupture,meantime,the new phase-change nanoparticles appeared and enlarged gradually.After LIFU irradiation,in both model of two-dimensional ultrasound and contrast-enhanced ultrasound, the echo of lipid nanoparticles was more significantly enhanced than before.Conclutions SL-NP was prepared successfully and could occur phase-change via heating and ultrasound radiation.PART II THE EXPERIMENTAL STUDY OF PHASE-CHANGE ULTRASOUND MOLECULAR PROBE PHYSICAL DESTRUCTING HEPATOCELLULAR CARCINOMA CELLObjective To explore the killing effect of SL-NP combined with LIFU on HepG2 cells.Methods NSL-NP and SL-NP were prepared by using film dispersion method;To observe the binding of SL-NP and NSL-NP with HepG2 cells used by the inverted fluorescence microscope, the cells were divided into targeted group (SL-NP group),non-targeted group (NSL-NP group) and blank control group(without L-NP group),each group was irradiated by LIFU(the parameters 3W,2min),and then flow cytometry detected the cell apoptosis and CCK-8 detected cell survival rate.Results The appearance of the prepared NSL-NP was opacified suspension,spherical,high density,uniform size,uniform dispersion and without accumulation,its physical characteristics was similar with SL-NP; the inverted fluorescence microscope discovered that SL-NP combined with HepG2 cells closely in targeted group,but in non-targeted group,NSL-NP rarely combined with HepG2 cells;After LIFU irradiation, targeted group had the highest cell apoptosis rate and the lowest survival rate, (95.86±1.96)%, (49.21±3.04)%,respectively.compared with other groups,the difference had statistical significance (P<0.05)Conclutions SL-NP combined with LIFU could promote death of HepG2 cells, inhibit its proliferation and be expected to be a new way to treat primary hepatocellular carcinoma..