The Research On The Method Of Opening Orifice And Xuan -fu Effect On Nasal Mucosa Channel Protein Of Rats With Rhinosinusitis

Purpose:1. Analysis of the understanding and treatment of "rhinosinusitis" ancient and modern literature and Combined with the ancient physicians understanding of "Xuan Fu" theory through literature research to find "tongqiao kaixuan method" theory and practice of foundation treatment of rhinosinusitis. 2.The technique of gene chip applied in analysis the aquaporin. what is the result of tongqiao kaixuan method effect on nasal mucosa in rhinosinusitis rat models.Methods:1.The research method of literature:System access to the understanding and treatment of ancient and modern literature of rhinosinusitis by Chinese medical classics, Chengdu University of Traditional Chinese Medicine library data, China HowNet CNKI database.Combination of successive dynasties about "Xuanfu" theory and "tongqiao kaixuan method"treatment theory of rhinosinusitis and mining theoretical basis of "tongqiao kaixuan method"treatment rhinosinusitis.2.The experimental research method:The rats are randomly divided into experimental group, model group and normal group.Improved method of experimental group and model group on the basis of Y.G el building.But normal groups do nothing.After the success of the building group respectively for sanhe tongqiao kaixuan Decoction low dose group, sanhe tongqiao kaixuan Decoction dose group, sanhe tongqiao kaixuan Decoction high dose group and Penicillin V potassium tablets to fill the stomach, to be put to death animals, nasal mucosal tissue.Application of the Agilent technologies in Santa Clara company gene chip, testing and analysis aquaporin in the rat nasal mucosa.Results:1.Literature research shows:Ancient doctors think sinusitis from Qingfei purging fire, evacuation of wind heat, replenishing qi to invigorate the spleen, clearing liver and gallbladder heat, eliminating phlegm for resuscitation for treatment rules, but also to using the theory of "tongqiao kaixuan method"the treatment of rhinosinusitis in clinic.Modern literature research shows, modern physicians through theoretical, experimental and clinical application of "tongqiao kaixuan method" the treatment of rhinosinusitis knowledge deepening gradually perfect and has been gradually rise to the theoretical level.2.The experimental results show:Penicillin V potassium tablets group and group of Chinese medicine treatment effect is similar.Traditional Chinese medicine group and penicillin V potassium tablets group compared with model group found that respectively get difference genes and pathways.Chinese medicine group were changed more than penicillin V potassium tablets group;Traditional Chinese medicine group was the upregulation of the expression of AQP1 in high dose group, agent, and the up regulation of AQP2, penicillin V potassium group only the up regulation of AQP 12b gene; four groups were downregulation of the AQP9 gene, low dose of Chinese medicine group and the down-regulation of AQP2, AQP12b, penicillin V potassium group and downregulation of AQP1, AQP2.Conclusions:1.In the literature,"tongqiao kaixuan method" for the treatment of rhinosinusitis are recordeds, clinical application of opening sweat pore method widely used in the treatment of rhinosinusitis."Tongqiao kaixuan method" for the treatment of rhinosinusitis have certain theory and practice foundation.2.Sanhe tongqiao kaixuan Decoction and Phenoxymethylpenicillin Potassium Tablets of rhinosinusitis with good therapeutic effect, but the expression of AQP related gene regulation mechanism is different, and the traditional Chinese medicine group effect is more widely, indicate that regulation of AQP related gene may be one of tongqiao kaixuan method in the treatment of rhinosinusitis biological mechanism.tongqiao kaixuan method could treat rhinosinusitis in rats and can provide new way for clinical treatment of rhinosinusitis.