The Delivery System Of Anti-FGFR3 PEGylation Immunoliposome And Characterization

Fibroblast growth factor receptor 3(FGFR3) is a member of the FGFR family, and it plays an important role in the regulation of cell proliferation and differentiation.It relates to the formation of many tumors and has become a potential target for cancer therapy.In this study, we designed a single chain fragment variable with a cysteine residue and coupled it with composition of liposome. So we prepared an tumorous target delivery of drugs.We amplified the target gene by PCR and constructed p ET-20b-Sc Fv-Cys recombinant plasmid with the method of molecular biology.The soluble protein expressed in Escherichia coli and purified by Ni affinity chromatography column.We obtain the target protein with the purity of 95% and more than 10 mg protein per liter.Then we coupled Sc Fv-Cys with mal PEG2000-DSPE under the condition of nitrogen and obtain Sc Fv-PEG2000-DSPE. We conducted the liposome and immunoliposome with the lipid hydration method and sonication.We detected liposome and immunoliposome with dynamic light scattering and electron microscope, and it showed that liposome had good particle size and morphology.In the end, the results showed that the immunoliposome had a significant target effect on the RT112 cells with high expression of FGFR3 and no significant target effect on the T24 cells as negative control through the experiment of the transfection of fluorescence labeled immunoliposome to the cells.In summary, we have successfully prepared a delivery system of anti-FGFR3 PEGylation immunoliposome and proved its effect.As a new target delivery system of drugs,it has hopeful application prospects in treatment of cancer and the development of drugs.