The Effect Of Hydrostatic High Pressure On The Immunogenicity Of Melanoma

Objective:Exploring the effect of hydrostatic high pressure on the immunogenicity of melanoma.The high hydrostatic pressure, liquid nitrogen freezing and thawing were used to break the murine B16 melanoma cells and then the cell suspension was mixed with Freund’s adjuvant to prepare vaccine for immunizing the mice. After immunization, the murine B16 melanoma cells were injected intravenously and subcutaneously. The immune results of the vaccines were elaluated, by comparing survival time of the mice, subcutaneous tumor volume, DTH experiments and the fluorescence imaging of tumors. Conclusion:Compared with the method of liquid nitrogen freeze-thaw broken, high hydrostatic pressure crushing cells has more effection on the immunogenicity of melanoma.