| Objective: To observe the effect of Eplerenone on current changes of voltagedependent potassium channel(Kv l.3 channel) in CD4~+ CD25~+ regulatory T(Treg) cells derived from spleen of chronic heart failure(CHF) Rat to provide evidence for immunological regulatory mechanism of the occurrence and development of CHF. Methods: Leftanterior descending coronary artery was ligated to induce CHF in SD rats. The plasma level of inflammatory related factor was detected by ELISA method. CD4~+ CD25~+ Treg cells were isolated from spleen of CHF Rat by magnetic-activated cell sorting. Whole-cell patch clamp technique was employed to check the current changes of Kv l.3 channel between CHF and control Rats. Results: Compared with the Control group, LVEF in the CHF group was decreased(84.5 ± 3.7% vs 39.3 ± 10%), and LVEDP was decreased(11.6 ± 1.4 mmHg vs 4.4 ± 0.3 mmHg), while plasma BNP level of CHF group was increased(366.2 ± 21.9 μg/L vs 562.0 ± 22.0 μg/L), all of which differences were statistical significance(P<0.01). For Masson’s trichrome staining, in the Control group myocardial cells were red with stripe clear, while a lot of blue collagen fibers instead of myocardial fibers in the CHF group. And compared with Control group, the CD4~+ CD25~+ Treg cell Kv1.3 potassium channel peak current density of CHF group was significantly increased(65.58 ± 6.36 pA/pF vs 272.65 ± 27.70 pA/pF, P<0.01), however, there was no significant difference for the membrane capacitance(2.07 ± 0.18 pF vs 1.67 ± 0.14 pF, P>0.05). Conclusion: The CD4~+ CD25~+ Treg cell Kv1.3 channel peak current density of the CHF group increased, compared with the Control group, perfusing CD4~+ CD25~+ Treg cells with Eplerenone in vitro, the Kv1.3 channel peak current density decreased, which was suggested CD4~+ CD25~+ Treg cells may be involved in the occurrence and development of CHF, Eplerenone may reduce the peak current density Kv1.3 channels to improve inflammatory reaction. |
PDF Full Text Request