Long Non-coding RNA SPRY4-IT1 Promotes The Proliferation And Invasion Of Human Glioma Cells Through Upregulation Of SKA2

Objective To investigate the role and related mechanism of Long non-coding RNA SPRY4-IT1 on proliferation and invasion of glioma cellsMethods Expression levels of Long non-coding RNA SPRY4-IT1 and SKA2 in glioma cells and glioma tissues were detected by quantitative real time polymerase chain reaction(q RT-PCR);we used lipofectamine2000-mediated small interfering RNAs(si RNAs) to knock down lnc RNA SPRY4-IT1 expression in u251 and u87 glioma cells; cell proliferation was examined by methyl thiazol tetrazolium(MTT)assay; the colony-forming capacity was verified by colony formation assay; the invasion and migration ability were detected by the means of transwell and Scratch wound assay. we demonstrated that the expression of SPRY4-IT1 was positively correlated to that of SKA2 in glioma tissues through analyzing Pearson correlation coefficient.Results we found that the expression of SPRY4-IT1 upregulated in glioma tissues(Mean±SD 12.14±1.35) than normal brain tissue(Mean±SD 0.89±0.09), the expression of SPRY4-IT1 was positively correlated to that of SKA2 in glioma tissues(r=0.382).After transfection of si RNA- SPRY4-IT1,as compared with control group, the cell viability detected by MTT assay and the clone numbers detected by colony formation assay both decreased.Inhibition of SPRY4-IT1 expression in u251 cells and u87 cells by RNAi significantly inhibited cell invasion and migration detected by the means of transwell and Scratch wound assay respectively.Conclusion the expression of SPRY4-IT1 increased in glioma tissues, whereas knockdown of SPRY4-IT1 inhibits human glioma cells growth, migration, and invasion. Moreover, SPRY4-IT1 may regulate the glioma malignant phenotype by SKA2. A further exploration of the functional and clinical implications of SPRY4-IT1 and its target SKA2 may facilitate the identification of novel therapeutic targets for glioma.