Expression And Regulation Of Tartrate-resistant Acid Phosphatase In The Granulosa Cells Of Women With Polycystic Ovary Syndrome

Polycystic ovary syndrome (PCOS) is a common complex endocrine condition. It is present in 12-21% of women of reproductive age. PCOS is a hyper-androgenic disorder associated with chronic oligo-anovulation, polycystic ovarian morphology, follicles degenerated at various degrees and other symptoms. On the other hand, it is difficult to deduce cause and effect.First of all, we compared the differences in protein expression in ovarian granulosae cells from normal women and patients with PCOS by antibody microarray technology. It showed that tartrate-resistant acid phosphatase (TRAP) differred significantly. Preliminary study in rat ovary and ovarian granulosa cells confined that the separated and cultured primary rat granulosa cells expressed TRAP and distributed in cytoplasm. In the rat ovary, TRAP was most active in the granular cells of the secondary follicle, and less active in later stage of the ovarian follicle. The results of TRAP activity assay showed that the activity of TRAP was significantly higher than that in the liver tissues, however, the TRAP activity in the granule cells of the three stage or mature follicles was significantly lower than that of the liver cells. It showed that the activity of TRAP in primary and secondary follicle granulosa cells was also very high, which indicated that the expression of TRAP in granulosa cells decreased or/and its release increased with the development of follicles. Secondly, the PCOS rat model was established by progesterone combined with HCG injection, and the difference of TRAP content and distribution in PCOS model group and control group were compared. The results found that in the 20 days HCG injected PCOS rats model group, contents of TRAP in primary and secondary follicles granulosa cells were significantly higher than those of control group. PCOS probably caused the reduced expression of TRAP in tertiary/mature follicle granulosa cells or/and excessive release. In addition,1,25-(OH)2-VD3, which promotes the synthesis and secretion of estrogen, has little effect on the activity of TRAP.The above results indicated that a certain amount of TRAP is synthesized and stored in granular cells in the secondary follicle stage. TRAP and the ROS produced by the TRAP will be gradually released into the follicle and play a role of degradation of collagen, layer associated protein and other effects including wall thinning of antral follicles and follicular, follicular rupture and the ovulation process. PCOS accelerates the synthesis and release of TRAP and ROS in granular cells, makes the follicular granular cells looses and the formation of follicular cavities in advance, which may be the cause of the polycystic ovarian changes.