Construction And Evaluation Of Delivery System Of Doxorubicin Imprinted Microcapsules-based Gate Control Effect

This paper reports the preparation of gate-controlled microcapsules toward the loading of the antitumor drug doxorubicin (DOX). The microcapsules, consisting completely of polysaccharides, were fabricated by deposition of oppositely charged chitosan and alginate on the surface of carboxylmethyl cellulose (CMC)-doped CaCO3 colloidal particles in a layer-by-layer fashion, followed by cross-linking with genipin, decomposition of the cores, loading of DOX and imprinting polymerization. The obtained imprinted microcapsules were evaluated by scanning electron microscope, exhibiting good dispersity and with diameter betweem 5-10μm. The loading and releaseing experiments of imprinted microcapsules indicated high loading capacity (up to 153.25μmol/g) and significant deceleration of the release rate. Moreover, in vitro cytotoxicity evaluation of imprinted microcapsules showed good cell viability over the entire concentration range from 20μg/mL to 100μg/mL. The results suggested that the imprinted microcapsules with narrow particle size distribution, high drug loading, low toxicity and slow release could be a potentially effective carrier for localised delivery of DOX.