AKT And Wnt11 Double Gene Combination Between Mice Bone Marrow Mesenchymal Stem Cell Transplantation For The Treatment Of Myocardial Infarction Mechanism

Objective: To explore the AKT overexpression of hydrogen peroxide(H2O2)between induced mouse bone marrow mesenchymal stem cells from oxidative damage and anti-apoptotic effects. Discussion Wnt11 overexpression of stem cells into cardiac cells into whether there is a facilitating role. Research on gene transfection double stem cells and cardiomyocytes after co-culture can be transformed into cardiomyocytes. Methods:Cultured stem cells, the best MOI lentivirus infection of stem cells. Cultured stem cells,gene transfection of AKT to the cell culture medium with different concentrations(25,50,100,200umol/L) of H2O2, culture 24 h establish oxidative damage models, each group contains AKT group, GFP control group, AKT inhibitor group. Stem cell flow cytometry proportion of apoptosis cells. Cultured stem cells, gene transfection under AKT,ischemia and hypoxia culture, and the control group. 24 h after cell flow cytometry Stem proportion of apoptosis cells. Stem cells were cultured for 3 days Wnt11 gene transfection,immunofluorescence assay whether myocardial cell marker protein positive cells were co-cultured myocardial cells were observed and recorded. Results: Stem cell apoptosis rate increased with H2O2 concentration rises upward trend. 25,50 umol group apoptosis rate lower than the control group(P <0.05), the apoptosis rate 100 umol group was slightly higher than the control group(P = 0.48), AKT inhibitor of apoptosis highest rate of model group(P <0.05). When ischemia and hypoxia, the apoptosis rate of the group of AKT overexpression was significantly lower than the control group(P<0.05).Immunofluorescence method can be observed in transfected Wnt11 group of myocardial cell marker protein positive cells. Cardiomyocyte-like cells co-cultured myocardial cells,expressed as cardiac cell-specific marker protein positive, the culture is set on the thirdday, the stem cells become the dominant cell. Conclusion: AKT overexpression can be resistance to oxidative stress,as well as apoptosis; Wnt11 genes promote stem cells into myocardial cells; double gene combined with stem cell treatment of myocardial infarction envisaged presence feasibility.